Evidence map›Paper›PMID 33852903›Full record

ArticleCell chemical biology2021

Inhibition of Staphylococcus aureus biofilm-forming functional amyloid by molecular tweezers.

Ravit Malishev, Nir Salinas, James Gibson, Angela Bailey Eden, Joel Mieres-Perez, Yasser B Ruiz-Blanco, Orit Malka, Sofiya Kolusheva, Frank-Gerrit Klärner, Thomas Schrader and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Review
  3. Anti-Heliyon · 2024
    Article
  4. Review
  5. Article
  6. Review
  7. Impact of cell-free supernatant ofFrontiers in veterinary science · 2023
    Article
  8. Article
  9. Article
  10. Article
  11. Anti-Biofilm Molecules Targeting Functional Amyloids.Antibiotics (Basel, Switzerland) · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 3 countries.

Ravit MalishevDepartment of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
Nir SalinasDepartment of Biology, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
James GibsonCenter for Biotechnology and Interdisciplinary Studies, Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Angela Bailey EdenCenter for Biotechnology and Interdisciplinary Studies, Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Joel Mieres-PerezDepartment of Computational Biochemistry, University of Duisburg-Essen, Universitätsstrasse 2, 45141 Essen, Germany.
Yasser B Ruiz-BlancoDepartment of Computational Biochemistry, University of Duisburg-Essen, Universitätsstrasse 2, 45141 Essen, Germany.
Orit MalkaDepartment of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
Sofiya KolushevaIlse Katz Institute for Nanotechnology, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
Frank-Gerrit KlärnerFaculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Thomas SchraderFaculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
Elsa Sanchez-GarciaDepartment of Computational Biochemistry, University of Duisburg-Essen, Universitätsstrasse 2, 45141 Essen, Germany.
Chunyu WangCenter for Biotechnology and Interdisciplinary Studies, Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Meytal LandauDepartment of Biology, Technion-Israel Institute of Technology, Haifa 3200003, Israel; European Molecular Biology Laboratory (EMBL), 22607 Hamburg, Germany.
Gal BitanDepartment of Neurology, David Geffen School of Medicine, Brain Research Institute, and Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA.
Raz JelinekDepartment of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105, Israel; Ilse Katz Institute for Nanotechnology, Ben Gurion University of the Negev, Beer Sheva 84105, Israel. Electronic address: razj@bgu.ac.il.
University of Duisburg-Essen · DEBen-Gurion University of the Negev · ILRensselaer Polytechnic Institute · USTechnion – Israel Institute of Technology · ILUniversity of California, Los Angeles · US

Funding

Investigation of the Effect of Structural Modifications of Tau on Assembly State and SeedingRF1AG054000 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BITAN, GAL · 2017 to 2021
$2.1M
Misfolded protein-clearance enhancers for Alzheimer's therapyR01AG050721 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BITAN, GAL · 2015 to 2019
$2.0M
NIA NIH HHS R01 AG050721NIA NIH HHS RF1 AG054000
6 · The paper itself

Abstract

Biofilms are rigid and largely impenetrable three-dimensional matrices constituting virulence determinants of various pathogenic bacteria. Here, we demonstrate that molecular tweezers, unique supramolecular artificial receptors, modulate biofilm formation of Staphylococcus aureus. In particular, the tweezers affect the structural and assembly properties of phenol-soluble modulin α1 (PSMα1), a biofilm-scaffolding functional amyloid peptide secreted by S. aureus. The data reveal that CLR01, a diphosphate tweezer, exhibits significant S. aureus biofilm inhibition and disrupts PSMα1 self-assembly and fibrillation, likely through inclusion of lysine side chains of the peptide. In comparison, different peptide binding occurs in the case of CLR05, a tweezer containing methylenecarboxylate units, which exhibits lower affinity for the lysine residues yet disrupts S. aureus biofilm more strongly than CLR01. Our study points to a possible role for molecular tweezers as potent biofilm inhibitors and antibacterial agents, particularly against untreatable biofilm-forming and PSM-producing bacteria, such as methicillin-resistant S. aureus.

Indexed as

AmyloidAnti-Bacterial AgentsBacterial ToxinsBiofilmsHemolysin ProteinsMicrobial Sensitivity TestsOptical TweezersStaphylococcus aureusAmyloidAnti-Bacterial AgentsBacterial ToxinsHemolysin Proteinsstaphylococcal alpha-toxinamyloid peptidesantibacterialbiofilmfunctional amyloidmolecular tweezerMRSAphenol-soluble modulinsPSMa1Staphylococcus aureus

Identifiers

PMID33852903
PMCPMC9377279
OpenAlexW3153598264

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.