Evidence map›Paper›PMID 33864885›Full record

ArticleMolecular and cellular endocrinology2021

Distinctive functioning of STARD1 in the fetal Leydig cells compared to adult Leydig and adrenal cells. Impact of Hedgehog signaling via the primary cilium.

Anbarasi Kothandapani, Michele Campaigne Larsen, Jinwoo Lee, Joan S Jorgensen, Colin R Jefcoate

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular endocrinology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Single-cell resolution uncovers neighboring cell subtypes that share steroidogenic capacity during fetal testis development.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  2. The role of primary cilia in the testis.Current opinion in endocrine and metabolic research · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Anbarasi KothandapaniDepartment of Comparative Biosciences, University of Wisconsin School of Veterinary Medicine, Madison, WI, 53706, USA.
Michele Campaigne LarsenDepartment of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705, USA.
Jinwoo LeeDepartment of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705, USA.
Joan S JorgensenDepartment of Comparative Biosciences, University of Wisconsin School of Veterinary Medicine, Madison, WI, 53706, USA.
Colin R JefcoateDepartment of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53705, USA. Electronic address: jefcoate@wisc.edu.
University of Wisconsin–Madison · US

Funding

Mediators for dynamic regulation of Star transcription in Leydig cellsR01HD090660 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI JEFCOATE, COLIN ROBERT, JORGENSEN, JOAN S · 2017 to 2021
$3.1M
NICHD NIH HHS R01 HD090660
6 · The paper itself

Abstract

STARD1 stimulates cholesterol transfer to mitochondrial CYP11A1 for conversion to pregnenolone. A cholesterol-binding START domain is guided by an N-terminal domain in a cell selective manner. Fetal and adult Leydig cells (FLC, ALC) show distinct Stard1 regulation. sm- FISH microscopy, which resolves individual molecules of Stard1 mRNA, shows uniformly high basal expression in each FLC. In ALC, in vivo, and cultured MA-10 cells, basal Stard1 expression is minimal. PKA activates loci asynchronously, with delayed splicing/export of 3.5 kb mRNA to mitochondria. After 60 min, ALC transition to an integrated mRNA delivery to mitochondria that is seen in FLC. Sertoli cells cooperate in Stard1 stimulation in FLC by delivering DHH to the primary cilium. There PTCH, SMO and cholesterol cooperate to release GLI3 to activate the Stard1 locus, probably by directing histone changes. ALC lack cilia. PKA then primes locus activation. FLC and ALC share similar SIK/CRTC/CREB regulation characterized for adrenal cells.

Indexed as

Alternative SplicingAnimalsCell LineChlorocebus aethiopsCiliaCOS CellsGene Expression Regulation, DevelopmentalHedgehog ProteinsHumansIn Situ Hybridization, FluorescenceLeydig CellsMalePhosphoproteinsProtein DomainsSignal TransductionSingle Molecule ImagingDHH protein, humanHedgehog ProteinsPhosphoproteinsSteroidogenic Acute Regulatory ProteinCholesterol traffickingCilium segregationFetal Leydig cellsPKA phosphorylationSmoothened/GLI transcriptionSTARD1

Identifiers

PMID33864885
PMCPMC13198195
OpenAlexW3156052754

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.