Evidence map›Paper›PMID 33865808›Full record

ArticleBiochimica et biophysica acta. Biomembranes2021

Site-selective labeling and electron paramagnetic resonance studies of human cannabinoid receptor CB

Alexei A Yeliseev, Kaeli Zoretich, Levi Hooper, Walter Teague, Lioudmila Zoubak, Kirk G Hines, Klaus Gawrisch

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimica et biophysica acta. Biomembranes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Special issue for Klaus Gawrisch.Biophysical journal · 2023
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Alexei A YeliseevNational Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: yeliseeva@mail.nih.gov.
Kaeli ZoretichNational Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892, USA.
Levi HooperNational Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892, USA.
Walter TeagueNational Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892, USA.
Lioudmila ZoubakNational Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892, USA.
Kirk G HinesNational Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892, USA.
Klaus GawrischNational Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address: klausg@mail.nih.gov.
National Institutes of Health · USNational Institute on Alcohol Abuse and Alcoholism · US

Funding

NMR INVESTIGATIONS OF CELL MEMBRANE STRUCTUREZ01AA000003 · NIAAA · NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM · PI GAWRISCH, KLAUS · 1993 to 2008
$5.4M
Intramural NIH HHS Z01 AA000003
6 · The paper itself

Abstract

Integral membrane G protein-coupled receptors (GPCR) regulate multiple physiological processes by transmitting signals from extracellular milieu to intracellular proteins and are major targets of pharmaceutical drug development. Since GPCR are inherently flexible proteins, their conformational dynamics can be studied by spectroscopic techniques such as electron paramagnetic resonance (EPR) which requires selective chemical labeling of the protein. Here, we developed protocols for selective chemical labeling of the recombinant human cannabinoid receptor CB

Indexed as

Electron Spin Resonance SpectroscopyCamphanesCyclohexanolsCysteineHumansMutagenesis, Site-DirectedProtein ConformationPyrazolesReceptor, Cannabinoid, CB2Receptors, CannabinoidReceptors, G-Protein-CoupledSpin Labels3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)-4-(3-hydroxypropyl)cyclohexanolCamphanesCNR2 protein, humanCyclohexanolsCysteinePyrazolesReceptor, Cannabinoid, CB2Receptors, CannabinoidReceptors, G-Protein-CoupledSpin LabelsSR 144528Cannabinoid receptor CB(2)Conformational transitionEPRGPCRSite-selective labeling

Identifiers

PMID33865808
PMCPMC8154700
OpenAlexW3153451767

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.