ArticlePLoS neglected tropical diseases2021
Metagenomic sequencing reveals viral abundance and diversity in mosquitoes from the Shaanxi-Gansu-Ningxia region, China.
Article in PLoS neglected tropical diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The application of metagenomics in the detection of arboviruses in mosquitoes (Diptera: Culicidade): a systematic review.Revista do Instituto de Medicina Tropical de Sao Paulo · 2025Pooled it
- Metavirome Analysis of Viruses Carried by Dairy Cows in Shaanxi, Gansu and Ningxia, China.Animals : an open access journal from MDPI · 2026Article
- Differences in metaviromes between Aedes aegypti and Aedes albopictus from sympatric areas on Hainan Island and the Leizhou Peninsula, China.Parasites & vectors · 2026Article
- Genomic Characterization of Dengue Virus in the Ningxia Hui Autonomous Region, China (2019 and 2023).Genome biology and evolution · 2026Article
- Mosquito viromes across England and Wales reveal hidden arbovirus signals and limited ecological structuring.Frontiers in microbiology · 2026Article
- Viral metagenomics in mosquitoes as potential vectors of arboviruses in the Colombian Caribbean: characterisation of a "core" regional RNA virome.Memorias do Instituto Oswaldo Cruz · 2026Article
- Bluetongue in China: Current Status of Viruses, Vectors, Detection Methods, and Vaccines.Transboundary and emerging diseases · 2026Review
- Phylogenetic insights into the transmission dynamics of arthropod-borne viruses.Nature reviews. Genetics · 2026Review
- The first discovery of the group IV GETV from Pangolin, China.Virulence · 2025Article
- Metagenomic Sequencing Reveals the Viral Diversity of Bactrian Camels in China.Microorganisms · 2025Article
- Unveiling viral diversity and dynamics in mosquitoes through metagenomic analysis in Guizhou Province, China.Infectious diseases of poverty · 2025Article
- Monitoring of Pathogens Carried by Imported Flies and Cockroaches at Shenzhen Ports.Tropical medicine and infectious disease · 2025Article
- Vector competence of EuropeanCurrent research in parasitology & vector-borne diseases · 2025Article
- An Exploration of the Viral Coverage of Mosquito Viromes Using Meta-Viromic Sequencing: A Systematic Review and Meta-Analysis.Microorganisms · 2024Review
- New Iflavirus Species Characterized from Mosquitoes Captured in the Sao Paulo Zoological Facilities.Microorganisms · 2024Article
- SegVir: Reconstruction of Complete Segmented RNA Viral Genomes from Metatranscriptomes.Molecular biology and evolution · 2024Article
- Metagenomic sequencing reveals viral diversity of mosquitoes from Shandong Province, China.Microbiology spectrum · 2024Article
- Tips and tools to obtain and assess mosquito viromes.Archives of microbiology · 2024Review
- Metatranscriptomic Sequencing of Medically Important Mosquitoes Reveals Extensive Diversity of RNA Viruses and Other Microbial Communities in Western Australia.Pathogens (Basel, Switzerland) · 2024Article
- Host influence on the eukaryotic virome of sympatric mosquitoes and abundance of diverse viruses with a broad host range.PloS one · 2024Article
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22 authors.
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Abstract
backgroundMosquitoes host and transmit numerous arthropod-borne viruses (arboviruses) that cause disease in both humans and animals. Effective surveillance of virome profiles in mosquitoes is vital to the prevention and control of mosquito-borne diseases in northwestern China, where epidemics occur frequently.
methodsMosquitoes were collected in the Shaanxi-Gansu-Ningxia region (Shaanxi Province, Gansu Province, and Ningxia Hui Autonomous Region) of China from June to August 2019. Morphological methods were used for taxonomic identification of mosquito species. High-throughput sequencing and metagenomic analysis were used to characterize mosquito viromes.
resultsA total of 22,959 mosquitoes were collected, including Culex pipiens (45.7%), Culex tritaeniorhynchus (40.6%), Anopheles sinensis (8.4%), Aedes (5.2%), and Armigeres subalbatus (0.1%). In total, 3,014,183 (0.95% of clean reads) viral sequences were identified and assigned to 116 viral species (including pathogens such as Japanese encephalitis virus and Getah virus) in 31 viral families, including Flaviviridae, Togaviridae, Phasmaviridae, Phenuiviridae, and some unclassified viruses. Mosquitoes collected in July (86 species in 26 families) showed greater viral diversity than those from June and August. Culex pipiens (69 species in 25 families) and Culex tritaeniorhynchus (73 species in 24 families) carried more viral species than Anopheles sinensis (50 species in 19 families) or Aedes (38 species in 20 families) mosquitoes.
conclusionViral diversity and abundance were affected by mosquito species and collection time. The present study elucidates the virome compositions of various mosquito species in northwestern China, improving the understanding of virus transmission dynamics for comparison with those of disease outbreaks.
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