Evidence mapPaperPMID 33917607Full record

ArticleMolecules (Basel, Switzerland)2021

β-Sitosterol Circumvents Obesity Induced Inflammation and Insulin Resistance by down-Regulating IKKβ/NF-κB and JNK Signaling Pathway in Adipocytes of Type 2 Diabetic Rats.

Selvaraj Jayaraman, Nalini Devarajan, Ponnulakshmi Rajagopal, Shyamaladevi Babu, Senthil Kumar Ganesan, Vishnu Priya Veeraraghavan, Chella Perumal Palanisamy, Bo Cui, Vijayalakshmi Periyasamy, Kirubhanand Chandrasekar

Open access · goldAbstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed, 1 pooled it
11.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 1 synthesis or guideline pooled it, 109 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Selvaraj JayaramanDepartment of Biochemistry, Saveetha Institute of Medical and Technical Sciences, Saveetha Dental College and Hospitals, Saveetha University, Chennai, Tamil Nadu 600077, India.ORCID 0000-0002-7726-1423
Nalini DevarajanCentral Research Laboratory, Meenakshi Ammal Dental College, Meenakshi Academy of Higher Education and Research, Maduravoyal, Chennai, Tamil Nadu 600095, India.
Ponnulakshmi RajagopalCentral Research Laboratory, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu 600078, India.
Shyamaladevi BabuDepartment of Biochemistry, Saveetha Institute of Medical and Technical Sciences, Saveetha Dental College and Hospitals, Saveetha University, Chennai, Tamil Nadu 600077, India.ORCID 0000-0001-7407-5273
Senthil Kumar GanesanStructural Biology & Bioinformatics Division, TRUE Campus, CSIR-Indian Institute of Chemical Biology, Kolkata 700032, India.ORCID 0000-0001-6040-4814
Vishnu Priya VeeraraghavanDepartment of Biochemistry, Saveetha Institute of Medical and Technical Sciences, Saveetha Dental College and Hospitals, Saveetha University, Chennai, Tamil Nadu 600077, India.
Chella Perumal PalanisamyState Key Laboratory of Biobased Materials and Green Papermaking, College of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Science, Jinan 250353, China.ORCID 0000-0002-3527-3477
Bo CuiState Key Laboratory of Biobased Materials and Green Papermaking, College of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Science, Jinan 250353, China.
Vijayalakshmi PeriyasamyDepartment of Biotechnology and Bioinformatics, Holy Cross College, Trichy, Tamil Nadu 620002, India.
Kirubhanand ChandrasekarDepartment of Anatomy, All India Institute of Medical Sciences, Nagpur, Maharashtra 440025, India.
Saveetha University · INMeenakshi Academy of Higher Education and Research · INQilu University of Technology · CNAll India Institute of Medical Sciences, Nagpur · INIndian Institute of Chemical Biology · IN

Funding

Science and Engineering Research Board ECR/2016/000415
6 · The paper itself

Abstract

β-sitosterol (SIT), the most abundant bioactive component of vegetable oil and other plants, is a highly potent antidiabetic drug. Our previous studies show that SIT controls hyperglycemia and insulin resistance by activating insulin receptor and glucose transporter 4 (GLUT-4) in the adipocytes of obesity induced type 2 diabetic rats. The current research was undertaken to investigate if SIT could also exert its antidiabetic effects by circumventing adipocyte induced inflammation, a key driving factor for insulin resistance in obese individuals. Effective dose of SIT (20 mg/kg b.wt) was administered orally for 30 days to high fat diet and sucrose induced type-2 diabetic rats. Metformin, the conventionally used antidiabetic drug was used as a positive control. Interestingly, SIT treatment restores the elevated serum levels of proinflammatory cytokines including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) to normalcy and increases anti-inflammatory adipocytokines including adiponectin in type 2 diabetic rats. Furthermore, SIT decreases sterol regulatory element binding protein-1c (SREBP-1c) and enhances Peroxisome Proliferator-activated receptor-γ (PPAR-γ) gene expression in adipocytes of diabetic rats. The gene and protein expression of c-Jun-N-terminal kinase-1 (JNK1), inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ) and nuclear factor kappa B (NF-κB) were also significantly attenuated in SIT treated groups. More importantly, SIT acts very effectively as metformin to circumvent inflammation and insulin resistance in diabetic rats. Our results clearly show that SIT inhibits obesity induced insulin resistance by ameliorating the inflammatory events in the adipose tissue through the downregulation of IKKβ/NF-κB and c-Jun-N-terminal kinase (JNK) signaling pathway.

Indexed as

Down-RegulationInsulin ResistanceAdipocytesAdipokinesAdipose TissueAnimalsBody WeightCytokinesDiabetes Mellitus, Type 2Diet, High-FatFeeding BehaviorI-kappa B KinaseInflammationInflammation MediatorsMaleMAP Kinase Signaling SystemAdipokinesCytokinesgamma-sitosterolI-kappa B KinaseInflammation MediatorsNF-kappa BPPAR gammaRNA, MessengerSitosterolsSterol Regulatory Element Binding Protein 1Sucroseadipokinesadipose tissueinflammationobesitysignaling pathwaystype-2 diabetes

Identifiers

PMID33917607
PMCPMC8038823
OpenAlexW3150964173

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.