Evidence map›Paper›PMID 33922274›Full record

ArticleAnimals : an open access journal from MDPI2021

RNA-Seq Reveals Function of Bta-miR-149-5p in the Regulation of Bovine Adipocyte Differentiation.

Hongfang Guo, Rajwali Khan, Sayed Haidar Abbas Raza, Syed Muhammad Suhail, Hamayun Khan, Sher Bahadar Khan, Ayman Hassan Abd El-Aziz, Linsen Zan

Open access · goldAbstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
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  8. Genes · 2025
    Article
  9. Article
  10. Article
  11. Review
  12. International journal of molecular sciences · 2023
    Article
  13. Editorial: Role of Non-Coding RNAs in Animals.Animals : an open access journal from MDPI · 2023
    Article
  14. Article
  15. Article
  16. Tumor-Suppressive and Oncogenic Roles of microRNA-149-5p in Human Cancers.International journal of molecular sciences · 2022
    Review
  17. Article
  18. Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 3 countries.

Hongfang GuoMedical College, Xuchang University, Xuchang, Henan 461000, China.ORCID 0000-0001-6032-9420
Rajwali KhanLivestock Management, Breeding and Genetics, The University of Agriculture, Peshawar 25120, Khyber Pakhtunkhwa, Pakistan.ORCID 0000-0001-5191-3457
Sayed Haidar Abbas RazaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.ORCID 0000-0002-0961-1911
Syed Muhammad SuhailLivestock Management, Breeding and Genetics, The University of Agriculture, Peshawar 25120, Khyber Pakhtunkhwa, Pakistan.
Hamayun KhanCollege of Veterinary Sciences, The University of Agriculture, Peshawar 25120, Khyber Pakhtunkhwa, Pakistan.
Sher Bahadar KhanCollege of Veterinary Sciences, The University of Agriculture, Peshawar 25120, Khyber Pakhtunkhwa, Pakistan.
Ayman Hassan Abd El-AzizDepartment of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.ORCID 0000-0002-8306-0011
Linsen ZanCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
The University of Agriculture, Peshawar · PKDamanhour University · EGNational Beef Cattle Industrial Technology System · CNNorthwest A&F University · CNXuchang University · CN

Funding

Key Research and Development Program of China 2018YFD0501700National Modern Agricultural Industry Special Program CARS-37National Science and Technology Support Projects 2015BAD03B04
6 · The paper itself

Abstract

Intramuscular fat is a real challenge for the experts of animal science to improve meat quality traits. Research on the mechanism of adipogenesis provides invaluable information for the improvement of meat quality traits. This study investigated the effect of bta-miR-149-5p and its underlying mechanism on lipid metabolism in bovine adipocytes. Bovine adipocytes were differentiated and transfected with bta-miR-149-5p mimics or its negative control (NC). A total of 115 DEGs including 72 upregulated and 43 downregulated genes were identified in bovine adipocytes. The unigenes and GO term biological processes were the most annotated unigene contributor parts at 80.08%, followed by cellular component at 13.4% and molecular function at 6.7%. The KEGG pathways regulated by the DEGs were PI3K-Akt signaling pathway, calcium signaling pathway, pathways in cancer, MAPK signaling pathway, lipid metabolism/metabolic pathway, PPAR signaling pathway, AMPK signaling pathway, TGF-beta signaling pathway, cAMP signaling pathway, cholesterol metabolism, Wnt signaling pathway, and FoxO signaling pathway. In addition to this, the most important reactome enrichment pathways were R-BTA-373813 receptor CXCR2 binding ligands CXCL1 to 7, R-BTA-373791 receptor CXCR1 binding CXCL6 and CXCL8 ligands, R-BTA-210991 basigin interactions, R-BTA-380108 chemokine receptors binding chemokines, R-BTA-445704 calcium binding caldesmon, and R-BTA-5669034 TNFs binding their physiological receptors. Furthermore, the expression trend of the DEGs in these pathways were also exploited. Moreover, the bta-miR-149-5p significantly (

Indexed as

bovine adipocytesbta-miR-149-5plipid metabolismsRNA-Seq

Identifiers

PMID33922274
PMCPMC8145242
OpenAlexW3153230150

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.