Evidence mapPaperPMID 33924243Full record

ArticleInternational journal of molecular sciences2021

Role of SIRT1 in Isoflurane Conditioning-Induced Neurovascular Protection against Delayed Cerebral Ischemia Secondary to Subarachnoid Hemorrhage.

Meizi Liu, Keshav Jayaraman, Tusar Giri, Gregory J Zipfel, Umeshkumar Athiraman

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Inhaled Anesthetics: Beyond the Operating Room.Journal of clinical medicine · 2024
    Review
  3. Review
  4. Conditioning-based therapeutics for aneurysmal subarachnoid hemorrhage - A critical review.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2024
    Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Meizi LiuDepartment of Anesthesiology, Washington University in Saint Louis, Saint Louis, MO 63110, USA.
Keshav JayaramanDepartment of Anesthesiology, Washington University in Saint Louis, Saint Louis, MO 63110, USA.
Tusar GiriDepartment of Anesthesiology, Washington University in Saint Louis, Saint Louis, MO 63110, USA.ORCID 0000-0003-1682-2300
Gregory J ZipfelDepartment of Neurological Surgery, Washington University in Saint Louis, Saint Louis, MO 63110, USA.
Umeshkumar AthiramanDepartment of Anesthesiology, Washington University in Saint Louis, Saint Louis, MO 63110, USA.ORCID 0000-0002-9086-0607
Washington University in St. Louis · US

Funding

TRAINING PROGRAM IN ANESTHESIOLOGY RESEARCHT32GM108539 · NIGMS · WASHINGTON UNIVERSITY · 2024 to 2025
$1.1M
Brain Aneurysm Foundation 17822McDonnell Center for Cellular and Molecular Neurobiology grant 26275INIGMS NIH HHS T32 GM108539
6 · The paper itself

Abstract

We recently reported that isoflurane conditioning provided multifaceted protection against subarachnoid hemorrhage (SAH)-induced delayed cerebral ischemia (DCI), and this protection was through the upregulation of endothelial nitric oxide synthase (eNOS). SIRT1, an NAD-dependent deacetylase, was shown to be one of the critical regulators of eNOS. The aim of our current study is to examine the role of SIRT1 in isoflurane conditioning-induced neurovascular protection against SAH-induced DCI. Mice were divided into four groups: sham, SAH, or SAH with isoflurane conditioning (with and without EX-527). Experimental SAH via endovascular perforation was performed. Anesthetic conditioning was performed with isoflurane 2% for 1 h, 1 h after SAH. EX-527, a selective SIRT1 inhibitor, 10 mg/kg was injected intraperitoneally immediately after SAH in the EX-527 group. SIRT1 mRNA expression and activity levels were measured. Vasospasm, microvessel thrombosis, and neurological outcome were assessed. SIRT1 mRNA expression was downregulated, and no difference in SIRT1 activity was noted after isoflurane exposure. Isoflurane conditioning with and without EX-527 attenuated vasospasm, microvessel thrombosis and improved neurological outcomes. Our data validate our previous findings that isoflurane conditioning provides strong protection against both the macro and micro vascular deficits induced by SAH, but this protection is likely not mediated through the SIRT1 pathway.

Indexed as

Ischemic PreconditioningNeuroprotectionAnimalsBrain IschemiaDisease Models, AnimalFluorescent Antibody TechniqueGene ExpressionIsofluraneMiceSirtuin 1Subarachnoid HemorrhageVasospasm, IntracranialIsofluraneSirt1 protein, mouseSirtuin 1aneurysmal subarachnoid hemorrhagedelayed cerebral ischemiaisoflurane conditioningSIRT1

Identifiers

PMID33924243
PMCPMC8074752
OpenAlexW3153378702

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.