Evidence map›Paper›PMID 33925224›Full record

ArticleInternational journal of molecular sciences2021

Isolation and Characterization of Human Colon Adenocarcinoma Stem-Like Cells Based on the Endogenous Expression of the Stem Markers.

Sergei A Koshkin, Olga V Anatskaya, Alexander E Vinogradov, Vladimir N Uversky, Guy W Dayhoff, Margarita A Bystriakova, Valery A Pospelov, Elena N Tolkunova

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
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  5. Rapid prediction and analysis of protein intrinsic disorder.Protein science : a publication of the Protein Society · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Sergei A KoshkinInstitute of Cytology of the Russian Academy of Science, 194064 St-Petersburg, Russia.
Olga V AnatskayaInstitute of Cytology of the Russian Academy of Science, 194064 St-Petersburg, Russia.ORCID 0000-0003-4538-8056
Alexander E VinogradovInstitute of Cytology of the Russian Academy of Science, 194064 St-Petersburg, Russia.ORCID 0000-0001-5540-8896
Vladimir N UverskyDepartment of Molecular Medicine and USF Health Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.ORCID 0000-0002-4037-5857
Guy W DayhoffDepartment of Chemistry, College of Art and Sciences, University of South Florida, Tampa, FL 33620, USA.
Margarita A BystriakovaInstitute of Cytology of the Russian Academy of Science, 194064 St-Petersburg, Russia.
Valery A PospelovInstitute of Cytology of the Russian Academy of Science, 194064 St-Petersburg, Russia.
Elena N TolkunovaInstitute of Cytology of the Russian Academy of Science, 194064 St-Petersburg, Russia.ORCID 0000-0003-1254-2619
Institute of Cytology · RUUniversity of South Florida · USThomas Jefferson University · US

Funding

The work was partially supported by the Institute of Cytology Director's Fund for S.A.K., E.N.T., M.A.B., V.A.P., O.V.A. and A.E.V.
6 · The paper itself

Abstract

backgroundCancer stem cells' (CSCs) self-maintenance is regulated via the pluripotency pathways promoting the most aggressive tumor phenotype. This study aimed to use the activity of these pathways for the CSCs' subpopulation enrichment and separating cells characterized by the OCT4 and SOX2 expression.

methodsTo select and analyze CSCs, we used the SORE6x lentiviral reporter plasmid for viral transduction of colon adenocarcinoma cells. Additionally, we assessed cell chemoresistance, clonogenic, invasive and migratory activity and the data of mRNA-seq and intrinsic disorder predisposition protein analysis (IDPPA).

resultsWe obtained the line of CSC-like cells selected on the basis of the expression of the OCT4 and SOX2 stem cell factors. The enriched CSC-like subpopulation had increased chemoresistance as well as clonogenic and migration activities. The bioinformatic analysis of mRNA seq data identified the up-regulation of pluripotency, development, drug resistance and phototransduction pathways, and the downregulation of pathways related to proliferation, cell cycle, aging, and differentiation. IDPPA indicated that CSC-like cells are predisposed to increased intrinsic protein disorder.

conclusionThe use of the SORE6x reporter construct for CSCs enrichment allows us to obtain CSC-like population that can be used as a model to search for the new prognostic factors and potential therapeutic targets for colon cancer treatment.

Indexed as

AdenocarcinomaAdultBiomarkers, TumorCell Culture TechniquesCell CycleCell Line, TumorCell MovementCell ProliferationColonic NeoplasmsFemaleGene Expression Regulation, NeoplasticHumansMaleMiddle AgedNeoplastic Stem CellsOctamer Transcription Factor-3Biomarkers, TumorOctamer Transcription Factor-3POU5F1 protein, humanSOX2 protein, humanSOXB1 Transcription Factorscancer stem-like cellscolon cancerintrinsic disorder predisposition analysismRNA seqOCT4SORE6xreporter

Identifiers

PMID33925224
PMCPMC8124683
OpenAlexW3158253770

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.