Evidence map›Paper›PMID 33925229›Full record

ArticleInternational journal of molecular sciences2021

Skeletal Muscle Proteomic Profile Revealed Gender-Related Metabolic Responses in a Diet-Induced Obesity Animal Model.

Manuela Moriggi, Sara Belloli, Pietro Barbacini, Valentina Murtaj, Enrica Torretta, Linda Chaabane, Tamara Canu, Silvia Penati, Maria Luisa Malosio, Antonio Esposito and 3 more

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 27 citations in OpenAlex.

  1. Relaxin-2: Shaping the Proteomic Landscape of Skeletal Muscle Physiology, Glucose Trafficking, and Mitochondrial Function in Rat.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
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  15. Ubiquitin-Specific Proteases (USPs) and Metabolic Disorders.International journal of molecular sciences · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 1 country.

Manuela MoriggiGastroenterology and Digestive Endoscopy Unit, IRCCS Policlinico San Donato, 20097 San Donato Milanese, Italy.ORCID 0000-0002-4718-0307
Sara BelloliInstitute of Molecular Bioimaging and Physiology, CNR, 20090 Segrate, Italy.ORCID 0000-0002-3079-3932
Pietro BarbaciniDepartment of Biomedical Sciences for Health, University of Milan, 20090 Segrate, Italy.ORCID 0000-0002-6230-8663
Valentina MurtajDepartment of Nuclear Medicine, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.
Enrica TorrettaIRCCS Istituto Ortopedico Galeazzi, 20161 Milan, Italy.ORCID 0000-0002-6374-3325
Linda ChaabaneExperimental Imaging Center, Preclinical Imaging Facility, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.ORCID 0000-0003-2782-9938
Tamara CanuExperimental Imaging Center, Preclinical Imaging Facility, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.
Silvia PenatiLaboratory of Pharmacology and Brain Pathology, Neuro Center, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.
Maria Luisa MalosioLaboratory of Pharmacology and Brain Pathology, Neuro Center, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy.ORCID 0000-0003-0626-3066
Antonio EspositoExperimental Imaging Center, Preclinical Imaging Facility, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.ORCID 0000-0002-1170-6266
Cecilia GelfiDepartment of Biomedical Sciences for Health, University of Milan, 20090 Segrate, Italy.
Rosa Maria MorescoInstitute of Molecular Bioimaging and Physiology, CNR, 20090 Segrate, Italy.ORCID 0000-0002-2771-9387
Daniele CapitanioDepartment of Biomedical Sciences for Health, University of Milan, 20090 Segrate, Italy.ORCID 0000-0001-7701-728X
Vita-Salute San Raffaele University · ITUniversity of Milan · ITIRCCS Humanitas Research Hospital · ITIRCCS Policlinico San Donato · ITIstituto Ortopedico Galeazzi · IT

Funding

Italian Ministry of University and Research (MUR) PRIN 201742SBXA
6 · The paper itself

Abstract

Obesity is a chronic, complex pathology associated with a risk of developing secondary pathologies, including cardiovascular diseases, cancer, type 2 diabetes (T2DM) and musculoskeletal disorders. Since skeletal muscle accounts for more than 70% of total glucose disposal, metabolic alterations are strictly associated with the onset of insulin resistance and T2DM. The present study relies on the proteomic analysis of gastrocnemius muscle from 15 male and 15 female C56BL/J mice fed for 14 weeks with standard, 45% or 60% high-fat diets (HFD) adopting a label-free LC-MS/MS approach followed by bioinformatic pathway analysis. Results indicate changes in males due to HFD, with increased muscular stiffness (Col1a1, Col1a2, Actb), fiber-type switch from slow/oxidative to fast/glycolytic (decreased Myh7, Myl2, Myl3 and increased Myh2, Mylpf, Mybpc2, Myl1), increased oxidative stress and mitochondrial dysfunction (decreased respiratory chain complex I and V and increased complex III subunits). At variance, females show few alterations and activation of compensatory mechanisms to counteract the increase of fatty acids. Bioinformatics analysis allows identifying upstream molecules involved in regulating pathways identified at variance in our analysis (Ppargc1a, Pparg, Cpt1b, Clpp, Tp53, Kdm5a, Hif1a). These findings underline the presence of a gender-specific response to be considered when approaching obesity and related comorbidities.

Indexed as

AnimalsChromatography, LiquidDiabetes Mellitus, Type 2Diet, High-FatDisease Models, AnimalFemaleGlucoseInsulinInsulin ResistanceMaleMiceMice, Inbred C57BLMuscle, SkeletalObesityOxidative StressProteomicsGlucoseInsulininsulin resistanceobesityproteomicssarcopeniaskeletal muscle

Identifiers

PMID33925229
PMCPMC8125379
OpenAlexW3158295466

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.