ReviewFrontiers in genetics2021
Electroporation-Mediated Genome Editing of Livestock Zygotes.
Review in Frontiers in genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells.Nature biotechnology · 2026Article
- Electroporation-mediated site-specific knock-in of a human coding sequence into the bovine ALB locus in embryos.BMC biotechnology · 2026Article
- Selection of two pronuclei zygotes before electroporation does not improve gene editing efficiency or reduce mosaicism in porcine embryos despite optimized timing and Hoechst staining.Veterinary world · 2026Article
- Efficient genome editing in a Mozambique tilapia cell line using CAS ribonucleoprotein complexes.Scientific reports · 2026Article
- Efficient gene editing of BMP15, GDF9, and MSTN-but not the imprinted CLPG gene-in goat embryos via electrotransfection and handmade cloning.Functional & integrative genomics · 2025Article
- Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle.Scientific reports · 2025Article
- Retrospect and prospect: reproductive technologies in beef cattle.Molecular biology reports · 2025Review
- CRISPR/Cas9-editing of PRNP in Alpine goats.Veterinary research · 2025Article
- Novel electroporation microchip with field constriction enhances transfection efficiency and survival rates of feline embryos.Scientific reports · 2024Article
- Comparison Between Electroporation at Different Voltage Levels and Microinjection to Generate Porcine Embryos with Multiple Xenoantigen Knock-Outs.International journal of molecular sciences · 2024Article
- Commercial perspectives: Genome editing as a breeding tool for health and well-being in dairy cattle.JDS communications · 2024Article
- Electroporation Delivery of Cas9 sgRNA Ribonucleoprotein-Mediated Genome Editing in Sheep IVF Zygotes.International journal of molecular sciences · 2024Article
- Protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes.STAR protocols · 2024Article
- Gene editing in livestock: innovations and applications.Animal reproduction · 2024Article
- Optimising Electroporation Condition for CRISPR/Cas-Mediated Knockout in Zona-Intact Buffalo Zygotes.Animals : an open access journal from MDPI · 2023Article
- Ablation ofPNAS nexus · 2023Article
- Perspectives in Genome-Editing Techniques for Livestock.Animals : an open access journal from MDPI · 2023Review
- Transgenesis and Genome Engineering: A Historical Review.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Electroporation-Mediated CRISPR/Cas9 Genome Editing in Rat Zygotes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- LincRNA#1 knockout alone does not affect polled phenotype in cattle heterozygous for the celtic POLLED allele.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The introduction of genome editing reagents into mammalian zygotes has traditionally been accomplished by cytoplasmic or pronuclear microinjection. This time-consuming procedure requires expensive equipment and a high level of skill. Electroporation of zygotes offers a simplified and more streamlined approach to transfect mammalian zygotes. There are a number of studies examining the parameters used in electroporation of mouse and rat zygotes. Here, we review the electroporation conditions, timing, and success rates that have been reported for mice and rats, in addition to the few reports about livestock zygotes, specifically pigs and cattle. The introduction of editing reagents at, or soon after, fertilization can help reduce the rate of mosaicism, the presence of two of more genotypes in the cells of an individual; as can the introduction of nuclease proteins rather than mRNA encoding nucleases. Mosaicism is particularly problematic in large livestock species with long generation intervals as it can take years to obtain non-mosaic, homozygous offspring through breeding. Gene knockouts accomplished
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.