Evidence map›Paper›PMID 33928554›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Study of Signal Transduction Pathways by Phospho-Protein Evaluation.

Manuela Capone, Alessio Mazzoni, Laura Maggi

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Manuela CaponeDepartment of Experimental and Clinical Medicine and DENOTHE Center, University of Florence, Firenze, Italy. manuela.capone@unifi.it.
Alessio MazzoniDepartment of Experimental and Clinical Medicine and DENOTHE Center, University of Florence, Firenze, Italy.
Laura MaggiDepartment of Experimental and Clinical Medicine and DENOTHE Center, University of Florence, Firenze, Italy.
University of Florence · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Flow cytometric evaluation of phosphorylation status of signal transduction molecules is a useful method to study T-cell signaling pathways. As mutations occurring in TCR complex molecules, common gamma chain family's cytokines, their receptors or molecules involved in these pathways can lead to severe immune system defects, the study of T-cell signal transduction can be applied to both basic and clinical/translational research areas. In the present chapter, we show two different protocols for the study of T- cell response to an antigen-like stimulus and to IL-2.

Indexed as

Flow CytometrySignal TransductionAnimalsCD4-Positive T-LymphocytesCells, CulturedHumansInterleukin-2PhosphoproteinsPhosphorylationReceptors, Antigen, T-CellResearch DesignTetradecanoylphorbol AcetateWorkflowIL2 protein, humanInterleukin-2PhosphoproteinsReceptors, Antigen, T-CellTetradecanoylphorbol AcetateCytokinesPhosphorylationSignaling pathwaysT cellsTCR

Identifiers

PMID33928554
OpenAlexW3157473921

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.