ArticleProceedings of the National Academy of Sciences of the United States of America2021
Hindbrain neuropore tissue geometry determines asymmetric cell-mediated closure dynamics in mouse embryos.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Quantitative Analysis of Cell and Tissue Shape During Mouse Cranial Neural Tube Closure.Bio-protocol · 2026Article
- Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis.Current biology : CB · 2026Article
- Discovering the unexpected: Insights into the dynamics of mouse neural tube closure revealed by time-lapse imaging.Developmental biology · 2026Review
- GPR161-GLI3 repressor signaling at cilia directs apical constriction and cell fate during cranial neural tube closure.Development (Cambridge, England) · 2025Article
- Supracellular actomyosin assemblies: master coordinators of development.Development (Cambridge, England) · 2025Review
- A lateral tension model for mouse cranial neural tube closure.bioRxiv : the preprint server for biology · 2025Article
- A single-cell atlas of spatial and temporal gene expression in the mouse cranial neural plate.eLife · 2025Article
- Quantifying mechanical forces during vertebrate morphogenesis.Nature materials · 2024Article
- A Lifeact-EGFP quail for studying actin dynamics in vivo.The Journal of cell biology · 2024Article
- Optical coherence tomography-guided Brillouin microscopy highlights regional tissue stiffness differences during anterior neural tube closure in the Mthfd1l murine mutant.Development (Cambridge, England) · 2024Article
- The surface ectoderm exhibits spatially heterogenous tension that correlates with YAP localisation during spinal neural tube closure in mouse embryos.Cells & development · 2023Article
- The cellular dynamics of neural tube formation.Biochemical Society transactions · 2023Article
- Synchronisation of apical constriction and cell cycle progression is a conserved behaviour of pseudostratified neuroepithelia informed by their tissue geometry.Developmental biology · 2023Article
- Morphological phenotyping after mouse whole embryo culture.Frontiers in cell and developmental biology · 2023Review
- Cannabidiol impairs neural tube closure in mouse whole embryo culture.Birth defects research · 2022Article
- Interplay between substrate rigidity and tissue fluidity regulates cell monolayer spreading.Soft matter · 2022Article
- Vision and retina evolution: How to develop a retina.IBRO neuroscience reports · 2022Article
- Leading-edge elongation by follower cell interruption in advancing epithelial cell sheets.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Pulsatile contractions and pattern formation in excitable actomyosin cortex.PLoS computational biology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Gap closure is a common morphogenetic process. In mammals, failure to close the embryonic hindbrain neuropore (HNP) gap causes fatal anencephaly. We observed that surface ectoderm cells surrounding the mouse HNP assemble high-tension actomyosin purse strings at their leading edge and establish the initial contacts across the embryonic midline. Fibronectin and laminin are present, and tensin 1 accumulates in focal adhesion-like puncta at this leading edge. The HNP gap closes asymmetrically, faster from its rostral than caudal end, while maintaining an elongated aspect ratio. Cell-based physical modeling identifies two closure mechanisms sufficient to account for tissue-level HNP closure dynamics: purse-string contraction and directional cell motion implemented through active crawling. Combining both closure mechanisms hastens gap closure and produces a constant rate of gap shortening. Purse-string contraction reduces, whereas crawling increases gap aspect ratio, and their combination maintains it. Closure rate asymmetry can be explained by asymmetric embryo tissue geometry, namely a narrower rostral gap apex, whereas biomechanical tension inferred from laser ablation is equivalent at the gaps' rostral and caudal closure points. At the cellular level, the physical model predicts rearrangements of cells at the HNP rostral and caudal extremes as the gap shortens. These behaviors are reproducibly live imaged in mouse embryos. Thus, mammalian embryos coordinate cellular- and tissue-level mechanics to achieve this critical gap closure event.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.