ArticleGenome biology2021
Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf.
Article in Genome biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 57 citations in OpenAlex.
- Distinct Mg²⁺ responses and species-specific transcriptional regulation of the magnesium transporter MGR2 in Saccharum officinarum and Saccharum spontaneum.BMC plant biology · 2026Article
- Regulation of chloroplast biogenesis and differentiation.Journal of experimental botany · 2026Review
- A red/blue optoswitch for temporal control of chloroplast transcription and biogenesis in Arabidopsis.Nature communications · 2026Article
- Modulation of SAL retrograde signalling promotes yield and water productivity responses in dynamic field environments.The New phytologist · 2026Article
- Article
- Apocarotenoid signaling regulates meristem activity and shapes shoot and root lateral organ formation in Arabidopsis.Plant physiology · 2025Article
- The highly allo-autopolyploid modern sugarcane genome and very recent allopolyploidization in Saccharum.Nature genetics · 2025Article
- Fine mapping and candidate gene mining of major QTLFrontiers in plant science · 2025Article
- Plastid retrograde signaling: A developmental perspective.The Plant cell · 2024Review
- Recent progress on mechanisms that allocate cellular space to plastids.Fundamental research · 2024Review
- The Function of Florigen in the Vegetative-to-Reproductive Phase Transition in and around the Shoot Apical Meristem.Plant & cell physiology · 2024Review
- Article
- Thylakoid ultrastructural variations in chlorophyll-deficient wheat: aberrations or structural acclimation?Planta · 2024Article
- Variability in Leaf Color Induced by Chlorophyll Deficiency: Transcriptional Changes in Bamboo Leaves.Current issues in molecular biology · 2024Article
- Recent advances in exploring transcriptional regulatory landscape of crops.Frontiers in plant science · 2024Review
- Jasmonates, gibberellins, and powdery mildew modify cell cycle progression and evoke differential spatiotemporal responses along the barley leaf.Journal of experimental botany · 2024Article
- A molecular framework for grain number determination in barley.Science advances · 2023Article
- De novo transcriptome assemblies of CBMC genomics · 2023Article
- Correlated retrograde and developmental regulons implicate multiple retrograde signals as coordinators of chloroplast development in maize.The Plant cell · 2022Article
- Mutations in the chloroplast inner envelope protein TIC100 impair and repair chloroplast protein import and impact retrograde signaling.The Plant cell · 2022Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe developmental gradient in monocot leaves has been exploited to uncover leaf developmental gene expression programs and chloroplast biogenesis processes. However, the relationship between the two is barely understood, which limits the value of transcriptome data to understand the process of chloroplast development.
resultsTaking advantage of the developmental gradient in the bread wheat leaf, we provide a simultaneous quantitative analysis for the development of mesophyll cells and of chloroplasts as a cellular compartment. This allows us to generate the first biologically-informed gene expression map of this leaf, with the entire developmental gradient from meristematic to fully differentiated cells captured. We show that the first phase of plastid development begins with organelle proliferation, which extends well beyond cell proliferation, and continues with the establishment and then the build-up of the plastid genetic machinery. The second phase is marked by the development of photosynthetic chloroplasts which occupy the available cellular space. Using a network reconstruction algorithm, we predict that known chloroplast gene expression regulators are differentially involved across those developmental stages.
conclusionsOur analysis generates both the first wheat leaf transcriptional map and one of the most comprehensive descriptions to date of the developmental history of chloroplasts in higher plants. It reveals functionally distinct plastid and chloroplast development stages, identifies processes occurring in each of them, and highlights our very limited knowledge of the earliest drivers of plastid biogenesis, while providing a basis for their future identification.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.