ArticleNeuron2021
A photoswitchable GPCR-based opsin for presynaptic inhibition.
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Article in Neuron, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59citing papers in PubMed
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59 citing papers in PubMed, 97 citations in OpenAlex.
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Authors and funding
24 authors at 5 institutions in 2 countries.
Bryan A CopitsWashington University Pain Center, Washington University School of Medicine, St. Louis, MO, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA. Electronic address: bcopits@wustl.edu.
Raaj GowrishankarCenter of Excellence in the Neurobiology of Addiction, Pain, and Emotion, Departments of Anesthesiology and Pain Medicine, and Pharmacology, University of Washington, Seattle, WA, USA.
Patrick R O'NeillDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA; Shirley and Stefan Hatos Center for Neuropharmacology, Semel Institute, Department of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA.
Jun-Nan LiWashington University Pain Center, Washington University School of Medicine, St. Louis, MO, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Kasey S GirvenCenter of Excellence in the Neurobiology of Addiction, Pain, and Emotion, Departments of Anesthesiology and Pain Medicine, and Pharmacology, University of Washington, Seattle, WA, USA.
Judy J YooWashington University Pain Center, Washington University School of Medicine, St. Louis, MO, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Xenia MeshikDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Kyle E ParkerDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Skylar M SpanglerDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Abigail J ElerdingCenter of Excellence in the Neurobiology of Addiction, Pain, and Emotion, Departments of Anesthesiology and Pain Medicine, and Pharmacology, University of Washington, Seattle, WA, USA.
Bobbie J BrownWashington University Pain Center, Washington University School of Medicine, St. Louis, MO, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Sofia E ShirleyCenter of Excellence in the Neurobiology of Addiction, Pain, and Emotion, Departments of Anesthesiology and Pain Medicine, and Pharmacology, University of Washington, Seattle, WA, USA.
Kelly K L MaWashington University Pain Center, Washington University School of Medicine, St. Louis, MO, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Alexis M VasquezDepartment of Pharmacology, University of California San Diego, San Diego, CA, USA.
M Christine StanderDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Vani KalyanaramanDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Sherri K VogtWashington University Pain Center, Washington University School of Medicine, St. Louis, MO, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Vijay K SamineniWashington University Pain Center, Washington University School of Medicine, St. Louis, MO, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Tommaso PatriarchiInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
Lin TianDepartment of Biochemistry and Molecular Medicine, University of California Davis, Davis, CA, USA.
N GautamDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA.
Roger K SunaharaDepartment of Pharmacology, University of California San Diego, San Diego, CA, USA.
Robert W GereauWashington University Pain Center, Washington University School of Medicine, St. Louis, MO, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA; Department of Neuroscience, Washington University School of Medicine, St. Louis, MO, USA.
Michael R BruchasDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA; Department of Neuroscience, Washington University School of Medicine, St. Louis, MO, USA; Center of Excellence in the Neurobiology of Addiction, Pain, and Emotion, Departments of Anesthesiology and Pain Medicine, and Pharmacology, University of Washington, Seattle, WA, USA. Electronic address: mbruchas@uw.edu.
Washington University in St. Louis · USUniversity of Washington · USUniversity of California, San Diego · USUniversity of California, Davis · USUniversity of Zurich · CH
Funding
University of Washington Center of Excellence in Opioid Addiction ResearchP30DA048736 · NIDA · UNIVERSITY OF WASHINGTON · PI Charles Chavkin · 2019 to 2026
$13.0MTRAINING IN THE MOLECULAR PHARMACOLOGY OF ABUSED DRUGST32DA007278 · NIDA · UNIVERSITY OF WASHINGTON · PI Susan Marie Ferguson, Paul E. M. Phillips · 1993 to 2026
$9.8MStructure and dynamics of G protein coupled receptor-G protein complexesR01GM083118 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KOBILKA, BRIAN K, SKINIOTIS, GEORGIOS · 2008 to 2023
$8.3MNext Generation Opto-GPCRs for Neuromodulatory ControlU01NS128537 · NINDS · UNIVERSITY OF WASHINGTON · PI Andre Berndt, Michael R. Bruchas · 2023 to 2026
$4.4MNext-gen Opto-GPCRs: spatiotemporal simulation of neuromodulator signalingR01MH111520 · NIMH · WASHINGTON UNIVERSITY · PI BRUCHAS, MICHAEL R., SUNAHARA, ROGER K · 2016 to 2018
$3.2MDeciphering Signaling and Cell Behavior with Subcellular OptogeneticsR35GM122577 · NIGMS · WASHINGTON UNIVERSITY · PI GAUTAM, NARASIMHAN · 2017 to 2021
$2.8MNociceptin receptor signaling and regulation of dopamine transmission in drug reward circuitryK01DA042219 · NIDA · WASHINGTON UNIVERSITY · PI O'NEILL, PATRICK ROSS · 2017 to 2021
$715kDEFINING THE NEURAL BASIS FOR CHRONIC BLADDER PAIN AND ITS ASSOCIATED COMORBID DISORDERSK01DK115634 · NIDDK · WASHINGTON UNIVERSITY · PI SAMINENI, VIJAY K · 2018 to 2022
$582kTrans-synaptic optogenetics: reversible temporal control of activity at defined synaptic connectionsR21DA049569 · NIDA · WASHINGTON UNIVERSITY · PI COPITS, BRYAN, O'NEILL, PATRICK ROSS · 2019 to 2020
$389kNIDA NIH HHS K01 DA042219NIDA NIH HHS P30 DA048736NIDA NIH HHS R21 DA049569NIDA NIH HHS T32 DA007278NIDDK NIH HHS K01 DK115634NIGMS NIH HHS R01 GM083118NIGMS NIH HHS R35 GM122577NIMH NIH HHS R01 MH111520NINDS NIH HHS U01 NS128537
Abstract
Optical manipulations of genetically defined cell types have generated significant insights into the dynamics of neural circuits. While optogenetic activation has been relatively straightforward, rapid and reversible synaptic inhibition has proven more elusive. Here, we leveraged the natural ability of inhibitory presynaptic GPCRs to suppress synaptic transmission and characterize parapinopsin (PPO) as a GPCR-based opsin for terminal inhibition. PPO is a photoswitchable opsin that couples to G
Indexed as
Neural InhibitionRewardSynaptic TransmissionAnimalsDopamineExocytosisFish Proteinsgamma-Aminobutyric AcidGlutamic AcidHEK293 CellsHeLa CellsHumansMaleMiceOptogeneticsPresynaptic TerminalsDopamineFish Proteinsgamma-Aminobutyric AcidGlutamic Acidparapinopsin protein, Ictalurus punctatusReceptors, G-Protein-CoupledRod Opsinschemogeneticsinhibitory opsinneuronal inhibitionoptogeneticssynaptic inhibition
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.