Evidence map›Paper›PMID 34022283›Full record

ArticleCancer letters2021

Disrupting interferon-alpha and NF-kappaB crosstalk suppresses IFITM1 expression attenuating triple-negative breast cancer progression.

Olivia K Provance, Eric S Geanes, Asona J Lui, Anuradha Roy, Sean M Holloran, Sumedha Gunewardena, Christy R Hagan, Scott Weir, Joan Lewis-Wambi

Open access · hybridAbstract read
In one paragraph

Article in Cancer letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Article
  6. Article
  7. PARP9-PARP13-PARP14 axis tunes colorectal cancer response to radiotherapy.Journal of experimental & clinical cancer research : CR · 2025
    Article
  8. Article
  9. Article
  10. Versatile function of NF-ĸB in inflammation and cancer.Experimental hematology & oncology · 2024
    Review
  11. NMolecular neurobiology · 2024
    Article
  12. Integrated Omics Reveal the Pathogenic Potential ofTransboundary and emerging diseases · 2024
    Article
  13. Review
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  15. Article
  16. Review
  17. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Olivia K ProvanceDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA.
Eric S GeanesDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA.
Asona J LuiDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, 66160, USA.
Anuradha RoyDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA; High Throughput Screening Laboratory, University of Kansas, Lawrence, KS, 66049, USA.
Sean M HolloranDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA; Department of Biochemistry, University of Kansas Medical Center, Kansas City, KS, 66160, USA.
Sumedha GunewardenaDepartment of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, 66160, USA.
Christy R HaganDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA; Department of Biochemistry, University of Kansas Medical Center, Kansas City, KS, 66160, USA; The University of Kansas Cancer Center, Kansas City, KS, 66160, USA.
Scott WeirDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA; The University of Kansas Cancer Center, Kansas City, KS, 66160, USA; The Institute for Advancing Medical Innovation, Kansas City, KS, 66160, USA.
Joan Lewis-WambiDepartment of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA; The University of Kansas Cancer Center, Kansas City, KS, 66160, USA. Electronic address: jlewis-wambi@kumc.edu.
The University of Kansas Cancer Center · USUniversity of Kansas · USUniversity of Kansas Medical Center · US

Funding

WOMEN'S CANCERS RESEARCH PROGRAMP30CA118100 · NCI · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Yolanda Sanchez · 2005 to 2026
$57.1M
Transgenic & Gene-Targeting Shared ResourceP30CA168524 · NCI · UNIVERSITY OF KANSAS MEDICAL CENTER · PI ROY A. JENSEN · 2012 to 2026
$40.1M
Synthetic Chemical Biology CoreP20GM113117 · NIGMS · UNIVERSITY OF KANSAS LAWRENCE · PI HEFTY, P SCOTT · 2016 to 2025
$23.9M
NRSA Training CoreTL1TR001864 · NCATS · YALE UNIVERSITY · PI CANTLEY, LLOYD G, EDELMAN, E. JENNIFER · 2016 to 2025
$9.9M
Using PCORnet to Expand the DS-CONNECT Cohort Through Healthcare System Recruitment, Incorporating Electronic Health Records, and Assessing Self-DeterminationU54HD090216 · NICHD · UNIVERSITY OF KANSAS LAWRENCE · PI COLOMBO, JOHN A. · 2016 to 2020
$6.2M
Molecular Regulation of Cell Development and Differentiation Phase III COBREP30GM122731 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI ABRAHAMSON, DALE R · 2017 to 2021
$5.6M
Immune Therapy for Brain MetastasisR01CA269286 · NCI · YALE UNIVERSITY · PI Lucia Beatrice Jilaveanu, Harriet M. Kluger · 2023 to 2026
$2.2M
Super resolution microscope for the imaging core facilityS10OD023625 · OD · UNIVERSITY OF KANSAS MEDICAL CENTER · PI NISHIMUNE, HIROSHI · 2019 to 2019
$987k
The new biology of estrogen action in aromatase resistance breast cancer cellsK01CA120051 · NCI · FOX CHASE CANCER CENTER · PI LEWIS-WAMBI, JOAN SUEDEE · 2007 to 2011
$628k
Role of IFN inducible transmembrane protein 1 in AI-resistant breast cancerF30CA203160 · NCI · UNIVERSITY OF KANSAS MEDICAL CENTER · PI LUI, ASONA J · 2016 to 2019
$117k
NCATS NIH HHS TL1 TR001864NCI NIH HHS F30 CA203160NCI NIH HHS K01 CA120051NCI NIH HHS P30 CA118100NCI NIH HHS P30 CA168524NCI NIH HHS R01 CA269286NICHD NIH HHS U54 HD090216NIGMS NIH HHS P20 GM113117NIGMS NIH HHS P30 GM122731NIH HHS S10 OD023625
6 · The paper itself

Abstract

Overexpression of interferon induced transmembrane protein-1 (IFITM1) enhances tumor progression in multiple cancers, but its role in triple-negative breast cancer (TNBC) is unknown. Here, we explore the functional significance and regulation of IFITM1 in TNBC and strategies to target its expression. Immunohistochemistry staining of a tissue microarray demonstrates that IFITM1 is overexpressed in TNBC samples which is confirmed by TCGA analysis. Targeting IFITM1 by siRNA or CRISPR/Cas9 in TNBC cell lines significantly inhibits proliferation, colony formation, and wound healing in vitro. Orthotopic mammary fat pad and mammary intraductal studies reveal that loss of IFITM1 reduces TNBC tumor growth and invasion in vivo. RNA-seq analysis of IFITM1/KO cells reveals significant downregulation of several genes involved in proliferation, migration, and invasion and functional studies identified NF-κB as an important downstream target of IFITM1. Notably, siRNA knockdown of p65 reduces IFITM1 expression and a drug-repurposing screen of FDA approved compounds identified parthenolide, an NFκB inhibitor, as a cytotoxic agent for TNBC and an inhibitor of IFITM1 in vitro and in vivo. Overall, our findings suggest that targeting IFITM1 by suppressing interferon-alpha/NFκB signaling represents a novel therapeutic strategy for TNBC treatment.

Indexed as

AdultAgedAnimalsAntigens, DifferentiationCell Line, TumorCell MovementCell ProliferationDown-RegulationFemaleGene Expression Regulation, NeoplasticHumansInterferon-alphaMiceMiddle AgedNF-kappa BSignal TransductionAntigens, DifferentiationInterferon-alphaleu-13 antigenNF-kappa BIFITM1InterferonNF-kappaBParthenolideTNBC

Identifiers

PMID34022283
PMCPMC8221017
OpenAlexW3162879125

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.