Evidence mapPaperPMID 34035375Full record

ArticleScientific reports2021

MicroRNA in combination with HER2-targeting drugs reduces breast cancer cell viability in vitro.

Lisa Svartdal Normann, Miriam Ragle Aure, Suvi-Katri Leivonen, Mads Haugland Haugen, Vesa Hongisto, Vessela N Kristensen, Gunhild Mari Mælandsmo, Kristine Kleivi Sahlberg

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 33 citations in OpenAlex.

  1. Upregulation of a CircularInternational journal of molecular sciences · 2026
    Article
  2. Review
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  4. Article
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  6. Review
  7. Article
  8. Article
  9. Article
  10. How MicroRNAs Command the Battle against Cancer.International journal of molecular sciences · 2024
    Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Review
  19. miRNA: A Promising Therapeutic Target in Cancer.International journal of molecular sciences · 2022
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Lisa Svartdal NormannDepartment of Research and Innovation, Vestre Viken Hospital Trust, P.O. Box 800, 3004, Drammen, Norway.
Miriam Ragle AureDepartment of Medical Genetics, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
Suvi-Katri LeivonenApplied Tumor Genomics Research Program, Medical Faculty, University of Helsinki, Helsinki, Finland.
Mads Haugland HaugenDepartment of Tumor Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway.
Vesa HongistoDivision of Toxicology, Misvik Biology, Turku, Finland.
Vessela N KristensenDepartment of Medical Genetics, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
Gunhild Mari MælandsmoDepartment of Tumor Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway.
Kristine Kleivi SahlbergDepartment of Research and Innovation, Vestre Viken Hospital Trust, P.O. Box 800, 3004, Drammen, Norway. Kristine.Sahlberg@vestreviken.no.
Oslo University Hospital · NOUniversity of Oslo · NOUniversity of Helsinki · FIVestre Viken Hospital Trust · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

HER2-positive (HER2 +) breast cancer patients that do not respond to targeted treatment have a poor prognosis. The effects of targeted treatment on endogenous microRNA (miRNA) expression levels are unclear. We report that responsive HER2 + breast cancer cell lines had a higher number of miRNAs with altered expression after treatment with trastuzumab and lapatinib compared to poorly responsive cell lines. To evaluate whether miRNAs can sensitize HER2 + cells to treatment, we performed a high-throughput screen of 1626 miRNA mimics and inhibitors in combination with trastuzumab and lapatinib in HER2 + breast cancer cells. We identified eight miRNA mimics sensitizing cells to targeted treatment, miR-101-5p, mir-518a-5p, miR-19b-2-5p, miR-1237-3p, miR-29a-3p, miR-29c-3p, miR-106a-5p, and miR-744-3p. A higher expression of miR-101-5p predicted better prognosis in patients with HER2 + breast cancer (OS: p = 0.039; BCSS: p = 0.012), supporting the tumor-suppressing role of this miRNA. In conclusion, we have identified miRNAs that sensitize HER2 + breast cancer cells to targeted therapy. This indicates the potential of combining targeted drugs with miRNAs to improve current treatments for HER2 + breast cancers.

Indexed as

Breast NeoplasmsCell Line, TumorCell ProliferationCell SurvivalFemaleGene Expression ProfilingGene Expression Regulation, NeoplasticHumansLapatinibMicroRNAsPrognosisSurvival AnalysisTrastuzumabUp-RegulationLapatinibMicroRNAsMIRN101 microRNA, humanTrastuzumab

Identifiers

PMID34035375
PMCPMC8149698
OpenAlexW3165178048

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.