ArticleMolecular biology of the cell2021
Stress fiber strain recognition by the LIM protein testin is cryptic and mediated by RhoA.
Article in Molecular biology of the cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 29 citations in OpenAlex.
- Apical spectrin organizes cortical actin filament bundles to pattern C. elegans cuticle ridges.PLoS genetics · 2026Article
- Mechanosensitive FHL2 tunes endothelial function via microtubule-actomyosin crosstalk.The EMBO journal · 2026Article
- RhoB supports rubella infection and impairs endothelial barrier integrity through downstream ROCK signaling.Cell communication and signaling : CCS · 2026Article
- Nuclear Mechanics and Nuclear Mechanotransduction in Cancer Cell Migration and Invasion.Biomolecules · 2026Review
- High-Altitude Hypoxic Preconditioning Attenuates Lipopolysaccharide-Induced Lung Injury and is Associated with Alveolar-Capillary Barrier Maintenance.Journal of inflammation research · 2026Article
- Regulation of tension-dependent localization of LATS1 and LATS2 to adherens junctions.PloS one · 2026Article
- Strained actin binding by the Prickle2 LIM domains and their regulation in the full-length protein.Molecular biology of the cell · 2026Article
- GSK3β-driven phosphorylation of ABLIM1 regulates its interactions with titin cardiac muscle.The Journal of general physiology · 2025Article
- Rho-GTPases subfamily: cellular defectors orchestrating viral infection.Cellular & molecular biology letters · 2025Review
- Force-activated zyxin assemblies coordinate actin nucleation and crosslinking to orchestrate stress fiber repair.Current biology : CB · 2025Article
- T cells use focal adhesions to pull themselves through confined environments.The Journal of cell biology · 2024Article
- Cracked actin filaments as mechanosensitive receptors.Biophysical journal · 2024Article
- Principles and regulation of mechanosensing.Journal of cell science · 2024Review
- The ability of the LIMD1 and TRIP6 LIM domains to bind strained f-actin is critical for their tension dependent localization to adherens junctions and association with the Hippo pathway kinase LATS1.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Tandem LIM domain-containing proteins, LIMK1 and LMO1, directly bind to force-bearing keratin intermediate filaments.Cell reports · 2024Article
- Force-activated zyxin assemblies coordinate actin nucleation and crosslinking to orchestrate stress fiber repair.bioRxiv : the preprint server for biology · 2024Article
- Article
- T cells Use Focal Adhesions to Pull Themselves Through Confined Environments.bioRxiv : the preprint server for biology · 2023Article
- Article
- Shining a light on RhoA: Optical control of cell contractility.The international journal of biochemistry & cell biology · 2023Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The actin cytoskeleton is a key regulator of mechanical processes in cells. The family of LIM domain proteins have recently emerged as important mechanoresponsive cytoskeletal elements capable of sensing strain in the actin cytoskeleton. The mechanisms regulating this mechanosensitive behavior, however, remain poorly understood. Here we show that the LIM domain protein testin is peculiar in that despite the full-length protein primarily appearing diffuse in the cytoplasm, the C-terminal LIM domains alone recognize focal adhesions and strained actin, while the N-terminal domains alone recognize stress fibers. Phosphorylation mutations in the dimerization regions of testin, however, reveal its mechanosensitivity and cause it to relocate to focal adhesions and sites of strain in the actin cytoskeleton. Finally, we demonstrate that activated RhoA causes testin to adorn stress fibers and become mechanosensitive. Together, our data show that testin's mechanoresponse is regulated in cells and provide new insights into LIM domain protein recognition of the actin cytoskeleton's mechanical state.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.