Evidence map›Paper›PMID 34041791›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2021

TbVps41 regulates trafficking of endocytic but not biosynthetic cargo to lysosomes of bloodstream forms of Trypanosoma brucei.

Srinivasan Ramakrishnan, Rodrigo P Baptista, Beejan Asady, Guozhong Huang, Roberto Docampo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Srinivasan RamakrishnanCenter for Tropical and Emerging Global Diseases, Athens, GA, USA.
Rodrigo P BaptistaCenter for Tropical and Emerging Global Diseases, Athens, GA, USA.
Beejan AsadyCenter for Tropical and Emerging Global Diseases, Athens, GA, USA.
Guozhong HuangCenter for Tropical and Emerging Global Diseases, Athens, GA, USA.
Roberto DocampoCenter for Tropical and Emerging Global Diseases, Athens, GA, USA.ORCID 0000-0003-4229-8784
Hospital for Tropical Diseases · VNUniversity of Georgia · US

Funding

The roles of polyphosphate and acidocalcisomes in Trypanosoma bruceiR01AI077538 · NIAID · UNIVERSITY OF GEORGIA · PI DOCAMPO, ROBERTO · 2009 to 2019
$3.8M
NIAID NIH HHS R01 AI077538
6 · The paper itself

Abstract

The bloodstream stage of Trypanosoma brucei, the causative agent of African trypanosomiasis, is characterized by its high rate of endocytosis, which is involved in remodeling of its surface coat. Here we present evidence that RNAi-mediated expression down-regulation of vacuolar protein sorting 41 (Vps41), a component of the homotypic fusion and vacuole protein sorting (HOPS) complex, leads to a strong inhibition of endocytosis, vesicle accumulation, enlargement of the flagellar pocket ("big eye" phenotype), and dramatic effect on cell growth. Unexpectedly, other functions described for Vps41 in mammalian cells and yeasts, such as delivery of proteins to lysosomes, and lysosome-related organelles (acidocalcisomes) were unaffected, indicating that in trypanosomes post-Golgi trafficking is distinct from that of mammalian cells and yeasts. The essentiality of TbVps41 suggests that it is a potential drug target.

Indexed as

EndocytosisAnimalsLysosomesOrganellesProtein TransportProtozoan ProteinsRNA InterferenceTrypanosoma brucei bruceiTrypanosomiasisVesicular Transport ProteinsProtozoan ProteinsVesicular Transport ProteinsacidocalcisomeendocytosisHOPSlysosomeTrypanosoma bruceiVps41

Identifiers

PMID34041791
PMCPMC8171815
OpenAlexW3164421806

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.