Evidence mapPaperPMID 34090331Full record

ArticleBMC molecular and cell biology2021

Effect of Voacamine upon inhibition of hypoxia induced fatty acid synthesis in a rat model of methyln-nitrosourea induced mammary gland carcinoma.

Lakhveer Singh, Manjari Singh, Shubham Rastogi, Anurag Choudhary, Dinesh Kumar, Ritu Raj, Mohd Nazam Ansari, Abdulaziz S Saeedan, Gaurav Kaithwas

Open access · goldAbstract read
In one paragraph

Article in BMC molecular and cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Review
  3. Regulation of Transactivation at C-TAD Domain of HIF-1Oxidative medicine and cellular longevity · 2022
    Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Lakhveer SinghDepartment of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, VidyaVihar, Raebareily Road, Lucknow, 226025, India.
Manjari SinghDepartment of Pharmaceutical Sciences, Assam University, Silchar, Assam, 788011, India.
Shubham RastogiDepartment of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, VidyaVihar, Raebareily Road, Lucknow, 226025, India.
Anurag ChoudharyDepartment of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, VidyaVihar, Raebareily Road, Lucknow, 226025, India.
Dinesh KumarCenter for Biomedical Research, Sanjay Gandhi Post Graduate Institute of Medical Science, Lucknow, India.
Ritu RajCenter for Biomedical Research, Sanjay Gandhi Post Graduate Institute of Medical Science, Lucknow, India.
Mohd Nazam AnsariDepartment of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
Abdulaziz S SaeedanDepartment of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
Gaurav KaithwasDepartment of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, VidyaVihar, Raebareily Road, Lucknow, 226025, India. gauravpharm@gmail.com.
Babasaheb Bhimrao Ambedkar University · INPrince Sattam Bin Abdulaziz University · SASanjay Gandhi Post Graduate Institute of Medical Sciences · INAssam University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn the present study, fatty acid synthesis is targeted to combat mammary gland carcinoma by activating prolyl hydroxylase-2 with Voacamine alone and in combination with Tamoxifen. It was hypothesized that the activation of prolyl hydroxylase-2 would inhibit the hypoxia-induced fatty acid synthesis and mammary gland carcinoma. Mammary gland carcinoma was induced with a single dose administration of N-methyl-N-nitrosourea (50 mg/kg,i.p.) and treatment with Voacamine and Tamoxifen 15 days after carcinogen administration.

resultsAt the end of the study, hemodynamic profiling of animals was recorded to assess the cardiotoxic potential of the drug. Blood serum was separated and subjected to nuclear magnetic resonance spectroscopy. Carmine staining and histopathology of mammary gland tissue were performed to evaluate the anti-angiogenic potential of the drug. The antioxidant potential of the drug was measured with antioxidant markers. Western blotting was performed to study the effect of the drug at the molecular level.

conclusionResults of the study have shown that Voacamine treatment stopped further decrease in body weight of experimental animals. The hemodynamic study evidenced that Voacamine at a low dose is safe in cardiac patients. Microscopic evaluation of mammary gland tissue documented the anti-angiogenic potential of Voacamine and Tamoxifen therapy. Perturbed serum metabolites were also restored to normal along with antioxidant markers. Immunoblotting of mammary gland tissue also depicted restoration of proteins of the hypoxic and fatty acid pathway. Conclusively, Voacamine and its combination with Tamoxifen activated prolyl hydroxylase-2 to combat mammary gland carcinoma.

Indexed as

AnimalsAntineoplastic Combined Chemotherapy ProtocolsAntioxidantsBody WeightCarcinomaComputer SimulationElectrocardiographyFatty AcidsFemaleHeart RateHypoxia-Inducible Factor-Proline DioxygenasesIbogaineMammary Glands, AnimalMammary Neoplasms, ExperimentalMetabolomeMethylnitrosoureaAntioxidantsEgln1 protein, ratFatty AcidsHypoxia-Inducible Factor-Proline DioxygenasesIbogaineMethylnitrosoureaTamoxifenvoacamineFatty acid synthaseHypoxia-inducible factor-1αMammary gland carcinomaProlyl hydroxylase-2Tamoxifen

Identifiers

PMID34090331
PMCPMC8180083
OpenAlexW3172042377

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.