Evidence map›Paper›PMID 34095414›Full record

ArticlePractical laboratory medicine2021

Comparison of the Japan Society of Clinical Chemistry reference method and CDC method for HDL and LDL cholesterol measurements using fresh sera.

Yuzo Kayamori, Masakazu Nakamura, Koji Kishi, Takashi Miida, Kunihiro Nishimura, Tomonori Okamura, Satoshi Hirayama, Hirotoshi Ohmura, Hiroshi Yoshida, Masumi Ai and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Practical laboratory medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 12 institutions in 1 country.

Yuzo KayamoriDepartment of Health Sciences, Faculty of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka City, Fukuoka, 812-8582, Japan.
Masakazu NakamuraDepartment of Preventive Cardiology, Lipid Reference Laboratory, National Cerebral and Cardiovascular Center, 6-1 Kishibe-Shimmachi, Suita, Osaka, 564-8565, Japan (retired in 2018).
Koji KishiBio-Reagent Material Development, Bio-Diagnostic Reagent Technology Center, Sysmex Corporation, 1-1-2, Murotani, Nishi-ku, Kobe, 651-2241, Japan.
Takashi MiidaDepartment of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Kunihiro NishimuraDepartment of Preventive Medicine and Epidemiologic Informatics, National Cerebral and Cardiovascular Center, 6-1 Kishibe-Shimmachi, Suita, Osaka, 564-8565, Japan.
Tomonori OkamuraDepartment of Preventive Medicine and Public Health, Keio University Graduate School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Satoshi HirayamaDepartment of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Hirotoshi OhmuraDepartment of Cardiovascular Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Hiroshi YoshidaDepartment of Laboratory Medicine, The Jikei University Kashiwa Hospital, 161-1 Kashiwashita, Kashiwa City, Ciba, 277-8567, Japan.
Masumi AiTokyo Medical and Dental University, Medical Hospital, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Akira TanakaNutrition Clinic, Kagawa Nutrition University, 3-9-21 Chiyoda, Sakado City, Saitama, 350-0288, Japan.
Hiroyuki SuminoDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan.
Masami MurakamiDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan.
Ikuo InoueDepartment of Endocrinology and Diabetes, Saitama Medical University, 38 Morohongo, Moroyama-machi, Iruma-gun, Saitama, 350-0495, Japan.
Tamio TeramotoTeikyo Academic Research Center, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Shinji YokoyamaDepartment of Food and Nutritional Sciences, Practice Center for Registered Dietitian, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan.
Juntendo University · JPGunma University · JPNational Cerebral and Cardiovascular Center · JPChubu University · JPJikei University Kashiwa hospital · JPKagawa Nutrition University · JPKeio University · JPKyushu University · JPSaitama Medical University · JPSysmex (Japan) · JPTeikyo University · JPTokyo Medical and Dental University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesIn 2009, the Japan Society of Clinical Chemistry (JSCC) recommended a reference method for the measurement of serum high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels. This automated method uses cholesterol esterase-cholesterol dehydrogenase to measure cholesterol levels in fractions obtained after ultracentrifugation and dextran sulfate/magnesium chloride precipitation. In the present study, using fresh samples, we compared the LDL-C and HDL-C levels measured using this method with those measured using the traditional Centers for Disease Control and Prevention (CDC)-beta-quantification (BQ) method.

designand methods: Using both the JSCC and CDC-BQ methods, LDL-C/HDL-C levels were measured in 47 non-diseased and 126 diseased subjects, whose triglyceride levels were lower than 11.29 ​mmol/L (1000 ​mg/dL).

resultsFor LDL-C, the equation of the line representing the correlation between the two methods was y ​= ​0.991x + 0.009 ​mmol/L; r ​= ​0.999; and Sy/x ​= ​0.025 ​mmol/L, where x is the mean LDL-C level measured using the CDC-BQ method. Similarly, for HDL-C, the equation of the line representing the correlation between the two methods was y ​= ​0.988x + 0.041 ​mmol/L, r ​= ​0.999, and Sy/x ​= ​0.019 ​mmol/L, where x is the mean HDL-C level measured using the CDC-BQ method.

conclusionsThe JSCC method agreed with the CDC-BQ method in cases of both non-diseased and diseased subjects, including those with dyslipidemia.

Indexed as

AK, Abell-Kendallapo, apolipoproteinBeta-quantificationBFr-C, bottom fraction-cholesterolBQ, beta-quantificationCDC, Centers for Disease Control and PreventionCD, cholesterol dehydrogenaseCHE, cholesterol esteraseCholesterol dehydrogenaseDM, n-dodecyl-β-maltopyranosideEDDA, ethylenediamine-N,N′-diacetic acidEDTA·2Na, ethylenediamine-N,N′,N′,N′-tetraacetic acid, disodium salt, dihydrateHDL-C, high-density lipoprotein-cholesterolHDL-cholesterolHEPES, 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acidHomogeneous assayLB, Liebermann-BurchardLDL-cholesterolLDL-C, low-density lipoprotein-cholesterolNIST, National Institute of Standards and TechnologyReference methodSRM, Standard Reference MaterialSyx, standard deviation of the regression lineTC, total cholesterolTG, triglycerides

Identifiers

PMID34095414
PMCPMC8145738
OpenAlexW3157464049

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.