Evidence mapPaperPMID 34099009Full record

ArticleMolecular brain2021

Cannabinoid receptors distribution in mouse cortical plasma membrane compartments.

Hajar Miranzadeh Mahabadi, Haseeb Bhatti, Robert B Laprairie, Changiz Taghibiglou

Open access · goldAbstract read
In one paragraph

Article in Molecular brain, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. The Healthy and Diseased Retina Seen through Neuron-Glia Interactions.International journal of molecular sciences · 2024
    Review
  6. Milk-based culture ofFrontiers in nutrition · 2024
    Article
  7. Review
  8. Article
  9. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Hajar Miranzadeh MahabadiDepartment of Anatomy, Physiology, Pharmacology; College of Medicine, University of Saskatchewan, 105 Wiggins Road, Health Sciences Bldg. Room GD30.5, Saskatoon, SK, S7N 5E5, Canada.
Haseeb BhattiDepartment of Anatomy, Physiology, Pharmacology; College of Medicine, University of Saskatchewan, 105 Wiggins Road, Health Sciences Bldg. Room GD30.5, Saskatoon, SK, S7N 5E5, Canada.
Robert B LaprairieCollege of Pharmacy and Nutrition, University of Saskatchewan, 105 Wiggins Road, Health Sciences Bldg. Room 3B36, Saskatoon, SK, S7N 5E5, Canada. robert.laprairie@usask.ca.
Changiz TaghibiglouDepartment of Anatomy, Physiology, Pharmacology; College of Medicine, University of Saskatchewan, 105 Wiggins Road, Health Sciences Bldg. Room GD30.5, Saskatoon, SK, S7N 5E5, Canada. changiz.taghibiglou@usask.ca.ORCID 0000-0002-4527-7404
University of Saskatchewan · CADalhousie University · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The type 1 and type 2 cannabinoid receptors (CB1 and CB2 receptors) are class A G protein-coupled receptors (GPCRs) that are activated by endogenous lipids called endocannabinoids to modulate neuronal excitability and synaptic transmission in neurons throughout the central nervous system (CNS), and inflammatory processes throughout the body. CB1 receptor is one of the most abundant GPCRs in the CNS and is involved in many physiological and pathophysiological processes, including mood, appetite, and nociception. CB2 receptor is primarily found on immunomodulatory cells of both the CNS and the peripheral immune system. In this study, we isolated lipid raft and non-lipid raft fractions of plasma membrane (PM) from mouse cortical tissue by using cold non-ionic detergent and sucrose gradient centrifugation to study the localization of CB1 receptor and CB2 receptor. Lipid raft and non-lipid raft fractions were confirmed by flotillin-1, caveolin-1 and transferrin receptor as their protein biomarkers. Both CB1 receptor and CB2 receptor were found in non-raft compartments that is inconsistent with previous findings in cultured cell lines. This study demonstrates compartmentalization of both CB1 receptor and CB2 receptor in cortical tissue and warrants further investigation of CB1 receptor and CB2 receptor compartmental distribution in various brain regions and cell types.

Indexed as

AnimalsCell Line, TumorCell MembraneCerebral CortexHumansMaleMembrane MicrodomainsMiceMice, Inbred C57BLReceptors, CannabinoidReceptors, CannabinoidLipid raftsType 1 cannabinoid receptorType 2 cannabinoid receptor

Identifiers

PMID34099009
PMCPMC8183067
OpenAlexW3172512378

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.