ArticleTransgenic research2021
Analysis of expression characteristics of soybean leaf and root tissue-specific promoters in Arabidopsis and soybean.
Article in Transgenic research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- The alfalfa AP2/ERF transcription factor MsCBF4 enhances frost tolerance in Arabidopsis thaliana.BMC plant biology · 2025Article
- A method for maintaining the release of co-suppression and maximally restoring the RDR6 expression.Plant cell reports · 2025Article
- Identification of a root-specific expression promoter in poplar and its application in genetic engineering for cadmium phytoremediation.Plant cell reports · 2025Article
- Construction and Validation of CRISPR/Cas Vectors for Editing theCurrent issues in molecular biology · 2024Article
- Identifying aPeerJ · 2024Article
- Cloning and functional analysis of the DXR gene and promoter region in Osmanthus fragrans var. semperflorens.Functional & integrative genomics · 2023Article
- Identification of the inducible activity in the promoter of the soybeanPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Genome-Wide Tissue-Specific Genes Identification for Novel Tissue-Specific Promoters Discovery in Soybean.Genes · 2023Article
- Overview of Repressive miRNA Regulation by Short Tandem Target Mimic (STTM): Applications and Impact on Plant Biology.Plants (Basel, Switzerland) · 2023Review
- High-Resolution Translatome Analysis Reveals Cortical Cell Programs During Early Soybean Nodulation.Frontiers in plant science · 2022Article
- Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal.Biodesign research · 2021Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The characterization of tissue-specific promoters is critical for studying the functions of genes in a given tissue/organ. To study tissue-specific promoters in soybean, we screened tissue-specific expressed genes using published soybean RNA-Seq-based transcriptome data coupled with RT-PCR analysis. We cloned the promoters of three genes, GmADR1, GmBTP1, and GmGER1, and constructed their corresponding β-Glucuronidase (GUS) promoter-GUS reporter vectors. We generated transgenic Arabidopsis plants and examined the expression patterns of these promoters by GUS staining and RT-PCR analysis. We also transformed the promoter-GUS reporter vectors into soybean to obtain hairy roots, and examined promoter expression by GUS staining. We found a root-specific expression pattern of GmADR1 and GmBTP1 in both Arabidopsis and soybean, and the promoter of GmGER1 showed a leaf-specific pattern in transgenic Arabidopsis plants. To test the potential utility of these promoters in soybean improvement by transgenic means, we used the GmADR1 promoter to drive expression of a salt resistance gene in soybean, GmCaM4, by generating transgenic soybean plants. We found that the transgenic plants had significantly enhanced salt tolerance compared to non-transformed wild-type, suggesting that introducing endogenous promoters by transgenic means can drive the expression of functional genes in specific tissues and organs in soybean.
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