ArticleProceedings of the National Academy of Sciences of the United States of America1988
Receptors for the host low density lipoproteins on the hemoflagellate Trypanosoma brucei: purification and involvement in the growth of the parasite.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 170 citations in OpenAlex.
- Transforming the chemotherapy of human African trypanosomiasis.Clinical microbiology reviews · 2025Review
- Anti-Molecules (Basel, Switzerland) · 2023Article
- How much (ATP) does it cost to build a trypanosome? A theoretical study on the quantity of ATP needed to maintain and duplicate a bloodstream-form Trypanosoma brucei cell.PLoS pathogens · 2023Article
- The role of invariant surface glycoprotein 75 in xenobiotic acquisition by African trypanosomes.Microbial cell (Graz, Austria) · 2023Article
- Multiple unbiased approaches identify oxidosqualene cyclase as the molecular target of a promising anti-leishmanial.Cell chemical biology · 2021Article
- Expression of a specific variant surface glycoprotein has a major impact on suramin sensitivity and endocytosis inFASEB bioAdvances · 2019Article
- Trypanosoma brucei CYP51: Essentiality and Targeting Therapy in an Experimental Model.PLoS neglected tropical diseases · 2016Article
- Review
- A MORN Repeat Protein Facilitates Protein Entry into the Flagellar Pocket of Trypanosoma brucei.Eukaryotic cell · 2015Article
- ATG24 Represses Autophagy and Differentiation and Is Essential for Homeostasy of the Flagellar Pocket in Trypanosoma brucei.PloS one · 2015Article
- Drug strategies targeting CYP51 in neglected tropical diseases.Chemical reviews · 2014Review
- Solanesyl diphosphate synthase, an enzyme of the ubiquinone synthetic pathway, is required throughout the life cycle of Trypanosoma brucei.Eukaryotic cell · 2014Article
- Echinococcus granulosus genomics: a new dawn for improved diagnosis, treatment, and control of echinococcosis.Parasite (Paris, France) · 2014Article
- Lipid synthesis in protozoan parasites: a comparison between kinetoplastids and apicomplexans.Progress in lipid research · 2013Review
- Scanning electron microscopy of the human low-density lipoprotein interaction with the tegument of Schistosoma mansoni.Parasitology research · 2011Article
- Molecular determinants of ciliary membrane localization of Trypanosoma cruzi flagellar calcium-binding protein.The Journal of biological chemistry · 2011Article
- Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions.PloS one · 2011Article
- Lipid metabolism in Trypanosoma brucei.Molecular and biochemical parasitology · 2010Review
- Cellular and molecular remodeling of the endocytic pathway during differentiation of Trypanosoma brucei bloodstream forms.Eukaryotic cell · 2010Article
- Composition and sensory function of the trypanosome flagellar membrane.Current opinion in microbiology · 2010Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The slender bloodstream form of Trypanosoma brucei shows receptor-mediated endocytosis of low density lipoprotein (LDL) particles of its hosts. We have purified the LDL receptor of this species nearly to homogeneity (about 1000-fold purification) and obtained monospecific polyclonal antibodies against it. As analyzed by NaDodSO4/polyacrylamide gel electrophoresis, the purified receptor consists of a single subunit, with an apparent molecular mass of 86 kDa. Its isoelectric point is 5.9. On the average, each cell exposes 52,000 copies of low-affinity receptors (Kd of 250 nM) and 1800 copies of high-affinity receptors (Kd of 5.7 nM). According to indirect en bloc immunolabeling of fixed parasites, the receptor appears to be localized to the flagellar pocket membrane and the flagellar membrane and to be completely absent from the rest of the pericellular membrane. LDL is required for optimal growth of the trypanosome in vitro: cell growth can be inhibited either by removal of LDL from the culture medium or by antibodies against the purified LDL receptors. In both cases, growth is restored by the addition of excess LDL.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.