Evidence map›Paper›PMID 34182477›Full record

ArticleIn vivo (Athens, Greece)

High Pathological Reproducibility of Diet-induced Atherosclerosis in Microminipigs

Masayoshi Otake, Hiroaki Kawaguchi, Satoko Enya, Akihisa Kangawa, Tadashi Koga, Kei Matsuo, Sohsuke Yamada, Md Mahfuzur Rahman, Naoki Miura, Masatoshi Shibata and 1 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Nanomedicine for Diagnosis and Treatment of Atherosclerosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
    Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Masayoshi OtakeSwine and Poultry Department, Shizuoka Prefectural Research Institute of Animal Industry, Swine and Poultry Research Center, Kikugawa, Japan; micropig@sp-exp.pref.shizuoka.jp.
Hiroaki KawaguchiDepartment of Hygiene and Health Promotion Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Satoko EnyaSwine and Poultry Department, Shizuoka Prefectural Research Institute of Animal Industry, Swine and Poultry Research Center, Kikugawa, Japan.
Akihisa KangawaSwine and Poultry Department, Shizuoka Prefectural Research Institute of Animal Industry, Swine and Poultry Research Center, Kikugawa, Japan.
Tadashi KogaShin Nippon Biomedical Laboratories, Ltd., Kagoshima, Japan.
Kei MatsuoDepartment of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Sohsuke YamadaDepartment of Pathology and Laboratory Medicine, Kanazawa Medical University, Kahoku, Japan.
Md Mahfuzur RahmanVeterinary Teaching Hospital, Joint faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
Naoki MiuraVeterinary Teaching Hospital, Joint faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
Masatoshi ShibataSwine and Poultry Department, Shizuoka Prefectural Research Institute of Animal Industry, Swine and Poultry Research Center, Kikugawa, Japan.
Akihide TanimotoDepartment of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan; akit09@m3.kufm.kagoshima-u.ac.jp.
Industrial Research Institute of Shizuoka Prefecture · JPKagoshima University · JPKagoshima University Hospital · JPKanazawa Medical University Hospital · JPShin Nippon Biomedical Laboratories (Japan) · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimThe reproducibility of athero - sclerotic lesions was evaluated after the production of cloned-microminipigs and their offspring. MATERIALS AND

methodsCloned-microminipig-parents were produced by microminipigsomatic cell nuclei. These parents were crossbred and delivered males (F1-offspring) were divided into two groups: normal chow diet (NcD)-fed and high-fat/high-cholesterol diet (HcD)-fed groups. One of the F1-offsprings was subjected to cloning, and delivered males (F1-clones) were fed with HcD. After 8 weeks, all animals were necropsied for patho - physiological studies compared to non-cloned-microminipigs.

resultsHcD-fed F1-offspring and F1-clones, but not NcD-fed F1-offspring, exhibited increased serum lipid levels and systemic atherosclerosis, which were comparable to those of HcD-fed non-cloned-microminipigs. Homogeneity of variance analysis demonstrated that standard deviation values of serum lipoprotein and aortic atherosclerosis area from HcD-fed animals decreased in F1-offspring and F1-clones.

conclusionHcD-induced atherogenesis was highly reproducible in F1-offsprings and F1-clones, indicating that the atherosclerosis-prone genomic background was preserved in the cloned-microminipigs, which can be used for studies on human atherosclerosis and related diseases.

Indexed as

AtherosclerosisAnimalsCholesterolCloning, MolecularHumansMaleReproducibility of ResultsTechnologyCholesterolAtherosclerosisclonemicrominipig™somatic cell nuclear transfer

Identifiers

PMID34182477
PMCPMC8286495
OpenAlexW3176949123

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.