ArticleJournal of orthopaedic surgery and research2021
Vitamin D inhibits TNF-α induced apoptosis of human nucleus pulposus cells through regulation of NF-kB signaling pathway.
Article in Journal of orthopaedic surgery and research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- TEAD4 and RXRA Regulate the Function of Nucleus Pulposus Cells in Intervertebral Disc Degeneration Via the TNF-α/NF-κB Pathway: An Integrated Analysis of Single-Cell RNA-Seq, Bulk RNA-Seq, and In Vitro Validation.Applied biochemistry and biotechnology · 2026Article
- Article
- Vitamin D promotes autophagy to inhibit LPS-induced lung injury via targeting cathepsin D.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Multi-Omics Analysis of the Immune Effect of the Engineered Exosome Drug Delivery System in Inducing Macrophage Apoptosis.Pharmaceutics · 2025Article
- 1,25(OH)₂D₃ inhibits ferroptosis in nucleus pulposus cells via VDR signaling to mitigate lumbar intervertebral disc degeneration.Scientific reports · 2025Article
- Exploring the anti-inflammatory potential of vitamin D in cardiometabolic diseases.Metabolism open · 2025Article
- Copper aggravated synaptic damage after traumatic brain injury by downregulating BNIP3-mediated mitophagy.Autophagy · 2025Article
- The interplay between childhood obesity and vitamin D deficiency: mechanisms and implications.Frontiers in pediatrics · 2025Review
- Tetrahedral framework nucleic acids-based delivery of microRNA-155 alleviates intervertebral disc degeneration through targeting Bcl-2/Bax apoptosis pathway.Cell proliferation · 2024Article
- Tanshinone IIA alleviates IL-1β-induced chondrocyte apoptosis and inflammation by regulating FBXO11 expression.Clinics (Sao Paulo, Brazil) · 2024Article
- Hypoxic Preconditional Engineering Small Extracellular Vesicles Promoted Intervertebral Disc Regeneration by Activating Mir-7-5p/NF-Κb/Cxcl2 Axis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Article
- Vitamin D as a Shield against Aging.International journal of molecular sciences · 2023Review
- Digoxin protects against intervertebral disc degeneration via TNF/NF-κB and LRP4 signaling.Frontiers in immunology · 2023Article
- Vitamin D and neurodegenerative diseases.Heliyon · 2023Review
- Chromobox homolog 4 overexpression inhibits TNF-α-induced matrix catabolism and senescence by suppressing activation of the NF-κB signaling pathway in nucleus pulposus cells.Acta biochimica et biophysica Sinica · 2022Article
- TNFα-induced abnormal activation of TNFR/NF-κB/FTH1 in endometrium is involved in the pathogenesis of early spontaneous abortion.Journal of cellular and molecular medicine · 2022Article
- The Role of Vitamin D in Supporting Health in the COVID-19 Era.International journal of molecular sciences · 2022Review
- 1,25(OH)Oxidative medicine and cellular longevity · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo observe the effects of vitamin D on the apoptotic human nucleus pulposus cells under tumor necrosis factor-α (TNF-α) treatment.
methodsThe gene expression data was downloaded from the NCBI Gene Expression Omnibus (GEO) database ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34095 ). Differentially expressed genes between degenerative disc and non-degenerative disc were performed by R software. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment analyses were performed using The Database for Annotation, Visualization and Integrated Discovery (DAVID). Then, the human nucleus pulposus tissue was harvested from 12 patients according to the modified Pfirrmann classification and human nucleus pulposus cells were obtained from digestion of herniated nucleus pulposus tissue. The collected nucleus pulposus cells were treated with different concentration of TNF-α, and cellular apoptosis was measured by flow cytometry. Then, human nucleus pulposus cells were divided into following groups: normal culture medium, TNF-α treated, TNF-α, and vitamin D-treated groups. Cellular apoptosis rate was quantified by flow cytometry. Protein expression of p-p65, p65, and IkBa was detected with western blot analysis.
resultsA total of 536 differentially expressed genes were identified through bioinformatic analysis. KEGG pathway revealed that NF-kB signaling pathway was involved in the process of disc degeneration. In the NP cell cultures, vitamin D significantly increased cell proliferation potency. Furthermore, vitamin D inhibited TNF-α induced apoptosis of human nucleus pulposus cells. Vitamin D reduced the phospho-NF-κB/p65 expression in the TNF-α-treated NP cells.
conclusionVitamin D can attenuate TNF-α-induced NP cells apoptosis through interfering with the NF-κB pathway.
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