Evidence mapPaperPMID 34200322Full record

ArticleInternational journal of molecular sciences2021

A New Ultrasensitive Bioluminescence-Based Method for Assaying Monoacylglycerol Lipase.

Matteo Miceli, Silvana Casati, Pietro Allevi, Silvia Berra, Roberta Ottria, Paola Rota, Bruce R Branchini, Pierangela Ciuffreda

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity.International journal of molecular sciences · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Matteo MiceliDipartimento di Scienze Biomediche e Cliniche "Luigi Sacco", Università degli Studi di Milano, Via G.B. Grassi 74, 20157 Milano, Italy.
Silvana CasatiDipartimento di Scienze Biomediche e Cliniche "Luigi Sacco", Università degli Studi di Milano, Via G.B. Grassi 74, 20157 Milano, Italy.ORCID 0000-0002-3865-0709
Pietro AlleviDipartimento di Scienze Biomediche, Chirurgiche e Odontoiatriche, Università degli Studi di Milano, Via della Commenda 10, 20122 Milano, Italy.
Silvia BerraDipartimento di Scienze Biomediche e Cliniche "Luigi Sacco", Università degli Studi di Milano, Via G.B. Grassi 74, 20157 Milano, Italy.ORCID 0000-0003-1151-7040
Roberta OttriaDipartimento di Scienze Biomediche e Cliniche "Luigi Sacco", Università degli Studi di Milano, Via G.B. Grassi 74, 20157 Milano, Italy.ORCID 0000-0003-3379-0582
Paola RotaDipartimento di Scienze Biomediche, Chirurgiche e Odontoiatriche, Università degli Studi di Milano, Via della Commenda 10, 20122 Milano, Italy.ORCID 0000-0001-5795-4989
Bruce R BranchiniDepartment of Chemistry, Connecticut College, New London, CT 06320, USA.
Pierangela CiuffredaDipartimento di Scienze Biomediche e Cliniche "Luigi Sacco", Università degli Studi di Milano, Via G.B. Grassi 74, 20157 Milano, Italy.ORCID 0000-0003-2227-1373
University of Milan · ITConnecticut College · US

Funding

Air Force Office of Scientific Research FA9550-18-1-0017Università degli Studi di Milano Progetto Piano Sviluppo Unimi - Linea 3 - Bando SEED
6 · The paper itself

Abstract

A novel bioluminescent Monoacylglycerol lipase (MAGL) substrate 6-O-arachidonoylluciferin, a D-luciferin derivative, was synthesized, physico-chemically characterized, and used as highly sensitive substrate for MAGL in an assay developed for this purpose. We present here a new method based on the enzymatic cleavage of arachidonic acid with luciferin release using human Monoacylglycerol lipase (

Indexed as

LuminescenceAnti-Anxiety AgentsBenzodioxolesBenzothiazolesBiological AssayEnzyme InhibitorsHumansLuciferasesMonoacylglycerol LipasesPiperidinesAnti-Anxiety AgentsBenzodioxolesBenzothiazolesD-luciferinEnzyme InhibitorsJZL 184LuciferasesMonoacylglycerol LipasesPiperidinesbioluminescencekinetic assaymonoacylglycerol lipasePLG2

Identifiers

PMID34200322
PMCPMC8200976
OpenAlexW3167424590

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.