Evidence map›Paper›PMID 34233746›Full record

ArticleStem cell research & therapy2021

Mesenchymal stem cell therapy ameliorates metabolic dysfunction and restores fertility in a PCOS mouse model through interleukin-10.

Rishi Man Chugh, Hang-Soo Park, Abdeljabar El Andaloussi, Amro Elsharoud, Sahar Esfandyari, Mara Ulin, Lale Bakir, Alshimaa Aboalsoud, Mohamed Ali, Dalia Ashour and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
13.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 77 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Review
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Review
  20. Protective Effects ofJournal of pharmacopuncture · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Rishi Man Chugh *Department of Surgery, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Hang-Soo Park *Department of Obstetrics and Gynecology, University of Chicago, 5841 S. Maryland Ave, Chicago, IL, 60637, USA.
Abdeljabar El Andaloussi *Department of Pathology, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Amro ElsharoudDepartment of Surgery, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Sahar EsfandyariDepartment of Surgery, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Mara UlinDepartment of Surgery, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Lale BakirDepartment of Obstetrics and Gynecology, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Alshimaa AboalsoudDepartment of Obstetrics and Gynecology, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Mohamed AliDepartment of Surgery, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Dalia AshourDepartment of Obstetrics and Gynecology, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Prosper IgboeliDepartment of Obstetrics and Gynecology, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Nahed IsmailDepartment of Pathology, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA.
Jan McAllisterDepartment of Pathology, Penn State Hershey College of Medicine, Hershey, PA, USA.
Ayman Al-HendyDepartment of Surgery, University of Illinois at Chicago, 820 South Wood Street, Chicago, IL, 60612, USA. aalhendy@BSD.Uchicago.edu.ORCID 0000-0002-6512-603X
University of Illinois Chicago · USAin Shams University · EGPenn State Milton S. Hershey Medical Center · USTanta University · EGUniversity of Chicago · US

Funding

Functional Analysis of PCOS Candidate GenesR01HD083323 · NICHD · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · PI MCALLISTER, JAN M, STRAUSS, JEROME F · 2016 to 2020
$2.7M
NICHD NIH HHS R01 HD083323
6 · The paper itself

Abstract

backgroundPolycystic ovary syndrome (PCOS) is the most common endocrine and metabolic disorder in reproductive-age women. Excessive inflammation and elevated androgen production from ovarian theca cells are key features of PCOS. Human bone marrow mesenchymal stem cells (BM-hMSC) and their secreted factors (secretome) exhibit robust anti-inflammatory capabilities in various biological systems. We evaluated the therapeutic efficacy of BM-hMSC and its secretome in both in vitro and in vivo PCOS models.

methodsFor in vitro experiment, we treated conditioned media from BM-hMSC to androgen-producing H293R cells and analyzed androgen-producing gene expression. For in vivo experiment, BM-hMSC were implanted into letrozole (LTZ)-induced PCOS mouse model. BM-hMSC effect in androgen-producing cells or PCOS model mice was assessed by monitoring cell proliferation (immunohistochemistry), steroidogenic gene expression (quantitative real-time polymerase chain reaction [qRT-PCR] and Western blot, animal tissue assay (H&E staining), and fertility by pup delivery.

resultsBM-hMSC significantly downregulate steroidogenic gene expression, curb inflammation, and restore fertility in treated PCOS animals. The anti-inflammatory cytokine interleukin-10 (IL-10) played a key role in mediating the effects of BM-hMSC in our PCOS models. We demonstrated that BM-hMSC treatment was improved in metabolic and reproductive markers in our PCOS model and able to restore fertility.

conclusionOur study demonstrates for the first time the efficacy of intra-ovarian injection of BM-hMSC or its secretome to treat PCOS-related phenotypes, including both metabolic and reproductive dysfunction. This approach may represent a novel therapeutic option for women with PCOS. Our results suggest that BM-hMSC can reverse PCOS-induced inflammation through IL-10 secretion. BM-hMSC might be a novel and robust therapeutic approach for PCOS treatment.

Indexed as

Mesenchymal Stem CellsMesenchymal Stem Cell TransplantationPolycystic Ovary SyndromeAnimalsFemaleFertilityHumansInterleukin-10MiceInterleukin-10Cell transplantationCytokinesInterleukinMesenchymal stem cells (MSCs)

Identifiers

PMID34233746
PMCPMC8261924
OpenAlexW3158162890

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.