Evidence map›Paper›PMID 34242576›Full record

ArticleCurrent biology : CB2021

Proximity labeling reveals non-centrosomal microtubule-organizing center components required for microtubule growth and localization.

Ariana D Sanchez, Tess C Branon, Lauren E Cote, Alexandros Papagiannakis, Xing Liang, Melissa A Pickett, Kang Shen, Christine Jacobs-Wagner, Alice Y Ting, Jessica L Feldman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current biology : CB, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed.

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  20. Apical PAR-3 caps orient the mitotic spindle inbioRxiv : the preprint server for biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ariana D SanchezDepartment of Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA.
Tess C BranonDepartment of Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA; Departments of Genetics and Chemistry, Stanford University, Stanford, CA, USA.
Lauren E CoteDepartment of Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA.
Alexandros PapagiannakisDepartment of Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA.
Xing LiangDepartment of Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
Melissa A PickettDepartment of Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA.
Kang ShenDepartment of Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
Christine Jacobs-WagnerHoward Hughes Medical Institute, Stanford University, Stanford, CA, USA; Department of Biology and ChEM-H Institute, Stanford University, Stanford, CA, USA.
Alice Y TingDepartment of Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA; Departments of Genetics and Chemistry, Stanford University, Stanford, CA, USA; Chan Zuckerberg Biohub, San Francisco, CA, USA.
Jessica L FeldmanDepartment of Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA. Electronic address: feldmanj@stanford.edu.

Funding

Supplement to Enhance Wellness and Resiliency in the Graduate EnvironmentT32GM007276 · NIGMS · STANFORD UNIVERSITY · PI MORRISON, ASHBY J. · 1985 to 2023
$32.9M
Mapping protein communication between organs in homeostasis and diseaseR01DK121409 · NIDDK · HARVARD MEDICAL SCHOOL · PI CARR, STEVEN A, MCMAHON, ANDREW P. · 2018 to 2022
$8.3M
Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Ann E. Rougvie · 2012 to 2026
$7.5M
Patterning dendritic branches with environmental and neuronal surface moleculesR01NS082208 · NINDS · STANFORD UNIVERSITY · PI KANG SHEN · 2013 to 2026
$4.1M
Mechanisms controlling microtubule organization during cell differentiationDP2GM119136 · NIGMS · STANFORD UNIVERSITY · PI FELDMAN, JESSICA LYNN · 2015 to 2015
$2.4M
Investigating the establishment, structure, and function of microtubule organizing centers in differentiated cells in vivoR01GM133950 · NIGMS · STANFORD UNIVERSITY · PI FELDMAN, JESSICA LYNN · 2020 to 2023
$1.3M
NIDDK NIH HHS R01 DK121409NIGMS NIH HHS DP2 GM119136NIGMS NIH HHS R01 GM133950NIGMS NIH HHS T32 GM007276NIH HHS P40 OD010440NINDS NIH HHS R01 NS082208
6 · The paper itself

Abstract

Microtubules are polarized intracellular polymers that play key roles in the cell, including in transport, polarity, and cell division. Across eukaryotic cell types, microtubules adopt diverse intracellular organization to accommodate these distinct functions coordinated by specific cellular sites called microtubule-organizing centers (MTOCs). Over 50 years of research on MTOC biology has focused mainly on the centrosome; however, most differentiated cells employ non-centrosomal MTOCs (ncMTOCs) to organize their microtubules into diverse arrays, which are critical to cell function. To identify essential ncMTOC components, we developed the biotin ligase-based, proximity-labeling approach TurboID for use in C. elegans. We identified proteins proximal to the microtubule minus end protein PTRN-1/Patronin at the apical ncMTOC of intestinal epithelial cells, focusing on two conserved proteins: spectraplakin protein VAB-10B/MACF1 and WDR-62, a protein we identify as homologous to vertebrate primary microcephaly disease protein WDR62. VAB-10B and WDR-62 do not associate with the centrosome and instead specifically regulate non-centrosomal microtubules and the apical targeting of microtubule minus-end proteins. Depletion of VAB-10B resulted in microtubule mislocalization and delayed localization of a microtubule nucleation complex ɣ-tubulin ring complex (γ-TuRC), while loss of WDR-62 decreased the number of dynamic microtubules and abolished γ-TuRC localization. This regulation occurs downstream of cell polarity and in conjunction with actin. As this is the first report for non-centrosomal roles of WDR62 family proteins, we expand the basic cell biological roles of this important disease protein. Our studies identify essential ncMTOC components and suggest a division of labor where microtubule growth and localization are distinctly regulated.

Indexed as

Caenorhabditis elegansMicrotubule-Organizing CenterMicrotubulesAnimalsCentrosomeCytoskeletal ProteinsMicrotubule-Associated ProteinsTubulinCytoskeletal ProteinsMicrotubule-Associated ProteinsTubulinC. elegansdifferentiationepithelial cellmicrotubulesMTOCncMTOCspectraplakinTurboIDWD40 repeat protein

Identifiers

PMID34242576
PMCPMC8478408

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.