Evidence mapPaperPMID 34281454Full record

ArticleBioengineered2021

Investigation on the expression regulation of RIPK1/RIPK3 in the retinal ganglion cells (RGCs) cultured in high glucose.

Sheng Gao, Xi Huang, Yi Zhang, Li Bao, Xiaoyue Wang, Meixia Zhang

Open access · goldAbstract read
In one paragraph

Article in Bioengineered, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
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  9. The role of regulated necrosis in diabetes and its complications.Journal of molecular medicine (Berlin, Germany) · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Sheng GaoDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu, China.
Xi HuangDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu, China.
Yi ZhangDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu, China.
Li BaoDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu, China.
Xiaoyue WangDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu, China.
Meixia ZhangDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu, China.
Sichuan University · CNWest China Hospital of Sichuan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Diabetic retinopathy (DR) represents the most typical complication of type 2 diabetes mellitus and one of the most primary oculopathy causing blindness. However, the mechanism of DR remains unknown. RIPK1/RIPK3, as homologous serine/threonine kinases, are key elements in mediating necroptosis and may have functions in DR development. To clarify the relationship between DR and RIPK1/RIPK3, this study established a model of apoptosis using high-glucose induced RGCs, which were treated with 7.5, 19.5, and 35 mM D-glucose for 12, 24, and 48 h, respectively. Subsequently, the expression of RIPK1/RIPK3 was determined and the protective effect of necrostatin-1 on RGCs injury induced by high glucose was explored. The results demonstrated that the expression of RIPK1 and RIPK3 in the cells was increased markedly following 12 h treatment with 19.5 mM D-glucose. Additionally, following an addition of 100 μM necrostatin-1 in 19.5 mM D-glucose medium for RGCs treatment 12 h, the protein expression of RIPK1 and RIPK3 was decreased markedly, and the number of Nissl bodies in cells was increased substantially. The findings of the present study indicated that high glucose could induce the expression of RIPK1/RIPK3, and necrostatin-1 could effectively protect RGCs from D-glucose-induced cell necrosis.

Indexed as

Gene Expression Regulation, EnzymologicAnimalsCell SeparationGlucoseImidazolesIndolesMiceMice, Inbred C57BLReceptor-Interacting Protein Serine-Threonine KinasesRetinal Ganglion CellsGlucoseImidazolesIndolesnecrostatin-1Receptor-Interacting Protein Serine-Threonine KinasesRipk1 protein, mouseRipk3 protein, mouseD-glucosediabetic retinopathyRGCsRIPK1/RIPK3

Identifiers

PMID34281454
PMCPMC8806785
OpenAlexW3183653550

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.