Evidence map›Paper›PMID 34294073›Full record

ArticleBMC cancer2021

Transcriptome sequencing and multi-plex imaging of prostate cancer microenvironment reveals a dominant role for monocytic cells in progression.

Stefano Mangiola, Patrick McCoy, Martin Modrak, Fernando Souza-Fonseca-Guimaraes, Daniel Blashki, Ryan Stuchbery, Simon P Keam, Michael Kerger, Ken Chow, Chayanica Nasa and 13 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors at 9 institutions in 2 countries.

Stefano MangiolaBioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
Patrick McCoyDepartment of Surgery, The University of Melbourne, Parkville, Victoria, Australia.
Martin ModrakInstitute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic.
Fernando Souza-Fonseca-GuimaraesUniversity of Queensland Diamantina Institute, Translational Research Institute, University of Queensland, Brisbane, QLD, Australia.
Daniel BlashkiThe Peter Doherty Institute for Infection and Immunity, Parkville, Victoria, Australia.
Ryan StuchberyDepartment of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia.
Simon P KeamSir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.
Michael KergerDepartment of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia.
Ken ChowDepartment of Surgery, The University of Melbourne, Parkville, Victoria, Australia.
Chayanica NasaFlow Cytometry Facility, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
Melanie Le PageFlow Cytometry Facility, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
Natalie ListerCancer Program, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Simon MonardFlow Cytometry Facility, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
Justin PetersEpworth Center of Cancer Research, Clayton, Victoria, Australia.
Phil DundeeEpworth Center of Cancer Research, Clayton, Victoria, Australia.
Scott G WilliamsSir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.
Anthony J CostelloDepartment of Surgery, The University of Melbourne, Parkville, Victoria, Australia.
Paul J NeesonSir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.
Bhupinder PalThe Olivia Newton-John Cancer Research Institute, Heidelberg, Melbourne, Australia.
Nicholas D HuntingtonCancer Program, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Niall M Corcoran *Department of Surgery, The University of Melbourne, Parkville, Victoria, Australia.
Anthony T Papenfuss *Bioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. papenfuss@wehi.edu.au.ORCID http://orcid.org/0000-0002-1102-8506
Christopher M Hovens *Department of Surgery, The University of Melbourne, Parkville, Victoria, Australia.
The Royal Melbourne Hospital · AUThe University of Melbourne · AUWalter and Eliza Hall Institute of Medical Research · AUAustralian Regenerative Medicine Institute · AUEpworth Hospital · AUCzech Academy of Sciences · CZOlivia Newton-John Cancer Wellness & Research Centre · AUPeter Doherty Institute · AUTranslational Research Institute · AU

Funding

National Health and Medical Research Council 1054618
6 · The paper itself

Abstract

backgroundProstate cancer is caused by genomic aberrations in normal epithelial cells, however clinical translation of findings from analyses of cancer cells alone has been very limited. A deeper understanding of the tumour microenvironment is needed to identify the key drivers of disease progression and reveal novel therapeutic opportunities.

resultsIn this study, the experimental enrichment of selected cell-types, the development of a Bayesian inference model for continuous differential transcript abundance, and multiplex immunohistochemistry permitted us to define the transcriptional landscape of the prostate cancer microenvironment along the disease progression axis. An important role of monocytes and macrophages in prostate cancer progression and disease recurrence was uncovered, supported by both transcriptional landscape findings and by differential tissue composition analyses. These findings were corroborated and validated by spatial analyses at the single-cell level using multiplex immunohistochemistry.

conclusionsThis study advances our knowledge concerning the role of monocyte-derived recruitment in primary prostate cancer, and supports their key role in disease progression, patient survival and prostate microenvironment immune modulation.

Indexed as

Gene Expression ProfilingTranscriptomeComputational BiologyDisease ProgressionHigh-Throughput Nucleotide SequencingHumansImmunohistochemistryImmunophenotypingKaplan-Meier EstimateMaleMolecular Sequence AnnotationMonocytesPrognosisProstatic NeoplasmsTumor MicroenvironmentBayesCAPRA-SCholesterolDeconvolutionDifferential gene expressionEpithelialFACSImmunohistochemistryMacrophagesMicroenvironmentMyeloidPDL1Prostate cancerTranscriptomics

Identifiers

PMID34294073
PMCPMC8296706
OpenAlexW3183180144

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.