Evidence map›Paper›PMID 34322757›Full record

ArticleBiochemical genetics2022

Circ_0007031 Silencing Inhibits Cell Proliferation and Induces Cell Apoptosis via Downregulating MELK at a miR-485-3p-Dependent Way in Colorectal Cancer.

Shengtian Su, Wenjing Lu, Jun Liu, Liping Li, Liang Liu, Xiaoju Li, Dan Ye, Zhigao Yu

Abstract read
PubMed Publisher
In one paragraph

Article in Biochemical genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Shengtian SuDepartment of Oncology, Xiantao First People's Hospital Affiliated to Yangtze University, No. 29 Middle Section of Mianzhou Avenue, Xiantao City, 433000, Hubei Province, China.
Wenjing LuDepartment of Oncology, Xiantao First People's Hospital Affiliated to Yangtze University, No. 29 Middle Section of Mianzhou Avenue, Xiantao City, 433000, Hubei Province, China.
Jun LiuDepartment of Oncology, Xiantao First People's Hospital Affiliated to Yangtze University, No. 29 Middle Section of Mianzhou Avenue, Xiantao City, 433000, Hubei Province, China.
Liping LiDepartment of Oncology, Xiantao First People's Hospital Affiliated to Yangtze University, No. 29 Middle Section of Mianzhou Avenue, Xiantao City, 433000, Hubei Province, China.
Liang LiuDepartment of Oncology, Xiantao First People's Hospital Affiliated to Yangtze University, No. 29 Middle Section of Mianzhou Avenue, Xiantao City, 433000, Hubei Province, China.
Xiaoju LiDepartment of Oncology, Xiantao First People's Hospital Affiliated to Yangtze University, No. 29 Middle Section of Mianzhou Avenue, Xiantao City, 433000, Hubei Province, China.
Dan YeDepartment of Oncology, Xiantao First People's Hospital Affiliated to Yangtze University, No. 29 Middle Section of Mianzhou Avenue, Xiantao City, 433000, Hubei Province, China.
Zhigao YuDepartment of Oncology, Xiantao First People's Hospital Affiliated to Yangtze University, No. 29 Middle Section of Mianzhou Avenue, Xiantao City, 433000, Hubei Province, China. yuzhigao840808@163.com.ORCID http://orcid.org/0000-0002-1690-8481
Yangtze University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Colorectal cancer (CRC) is a malignant cancer with an increasing incidence. Circular RNA (circRNA) is recently found to participate in the regulation of CRC progression. However, the role of circ_0007031 in CRC malignant progression remains elusive. 50 CRC patients were implicated in this study. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the RNA expression of circ_0007031, microRNA-485-3p (miR-485-3p) and maternal embryonic leucine zipper kinase (MELK). Western blot analysis was conducted to determine protein expression. Cell viability and proliferation were demonstrated by cell counting kit-8 and 5-Ethynyl-29-deoxyuridine (EdU) assays, respectively. Cell cycle and apoptosis were investigated by flow cytometry analysis. The interaction among circ_0007031, miR-485-3p and MELK was predicted by online databases, and identified by dual-luciferase reporter assay. Mouse model assay was conducted to reveal the effect of circ_0007031 on tumor formation in vivo. Circ_0007031 and MELK expression were obviously increased, while miR-485-3p expression was decreased in CRC tissues and cells compared with normal colorectal tissues or cells. Circ_0007031 knockdown repressed proliferation, whereas induced cell arrest at G0/G1 phase and apoptosis. On the opposite, circ_0007031 overexpression promoted cell proliferation and induced cell arrest at S phase. Additionally, miR-485-3p inhibitors attenuated circ_0007031 silencing-mediated CRC cell malignancy. MiR-485-3p was unveiled to regulate CRC cell processes via targeting MELK. Circ_0007031 controlled MELK expression via interacting with miR-485-3p. Furthermore, circ_0007031 contributed to tumor formation in vivo. Circ_0007031 knockdown repressed CRC malignant progression by reducing MELK expression through associating with miR-485-3p, suggesting that circ_0007031 was a potential target for the therapy of CRC.

Indexed as

Colorectal NeoplasmsMicroRNAsAnimalsApoptosisCell ProliferationHumansMiceProtein Serine-Threonine KinasesRNA, CircularMELK protein, humanMicroRNAsMIRN485 microRNA, humanProtein Serine-Threonine KinasesRNA, CircularCirc_0007031CRCMELKmiR-485-3p

Identifiers

PMID34322757
OpenAlexW3185364757

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.