Evidence map›Paper›PMID 34382189›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Low Input Targeted Chromatin Capture (Low-T2C).

Ilias Boltsis, Karol Nowosad, Rutger W W Brouwer, Przemko Tylzanowski, Wilfred F J van IJcken, Danny Huylebroeck, Frank Grosveld, Petros Kolovos

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Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 4 countries.

Ilias BoltsisDepartment of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.
Karol NowosadDepartment of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.
Rutger W W BrouwerDepartment of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.
Przemko TylzanowskiDepartment of Biochemistry and Molecular Biology, Medical University of Lublin, Lublin, Poland.
Wilfred F J van IJckenDepartment of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.
Danny HuylebroeckDepartment of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.
Frank GrosveldDepartment of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands. f.grosveld@erasmusmc.nl.
Petros KolovosDepartment of Molecular Biology and Genetics, Democritus University of Thrace, University Campus Dragana, Alexandroupolis, Greece.
Erasmus MC · NLDemocritus University of Thrace · GRMedical University of Lublin · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Targeted chromatin capture (T2C) is a 3C-based method and is used to study the 3D chromatin organization, interactomes and structural changes associated with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.

Indexed as

Chromatin Assembly and DisassemblyChromatinChromosome MappingComputational BiologyGene Expression RegulationGene LibraryGenomicsReproducibility of ResultsChromatinChromatinChromatin conformation captureC-technologiesGenome architectureT2CThree-dimensional (3D) organizationTopologically associating domains (TADs)

Identifiers

PMID34382189
OpenAlexW3191407478

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.