Evidence map›Paper›PMID 34385369›Full record

ArticleScience (New York, N.Y.)2021

Spatial transcriptomics of planktonic and sessile bacterial populations at single-cell resolution.

Daniel Dar, Nina Dar, Long Cai, Dianne K Newman

Open access · greenAbstract read
In one paragraph

Article in Science (New York, N.Y.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 188 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
188citing papers in PubMed, 1 pooled it
16.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

188 citing papers in PubMed, 1 synthesis or guideline pooled it, 279 citations in OpenAlex.

  1. Pooled it
  2. From predicted bacteriocins to ecological function.Current research in microbial sciences · 2026
    Review
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  5. Article
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  10. Fast, StreamlinedChemical & biomedical imaging · 2026
    Article
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  12. Article
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  19. A Guide for Spatial Omics Technologies: Innovation, Evaluation, and Application.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  20. Article

128 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Daniel DarDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.ORCID 0000-0002-6650-5488
Nina DarDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Long CaiDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA. dkn@caltech.edu lcai@caltech.edu.ORCID 0000-0002-7154-5361
Dianne K NewmanDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA. dkn@caltech.edu lcai@caltech.edu.ORCID 0000-0003-1647-1918
California Institute of Technology · US

Funding

Biological mechanisms and consequences of efficient extracellular electron transfer in Pseudomonas aeruginosaR01AI127850 · NIAID · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI Dianne K Newman · 2017 to 2026
$5.6M
Testing the hypothesis that microbial energetic hijacking of the CF immune response selects for specific pathogens during lung function decline- Diversity SupplementR01HL152190 · NHLBI · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI NEWMAN, DIANNE K · 2020 to 2023
$2.0M
NHLBI NIH HHS R01 HL152190NIAID NIH HHS R01 AI127850
6 · The paper itself

Abstract

Capturing the heterogeneous phenotypes of microbial populations at relevant spatiotemporal scales is highly challenging. Here, we present par-seqFISH (parallel sequential fluorescence in situ hybridization), a transcriptome-imaging approach that records gene expression and spatial context within microscale assemblies at a single-cell and molecule resolution. We applied this approach to the opportunistic pathogen

Indexed as

TranscriptomeBiofilmsFimbriae ProteinsFlagellinGene Expression ProfilingGene Expression Regulation, BacterialIn Situ Hybridization, FluorescencePhenotypePlanktonPseudomonas aeruginosaPyocinsRNA, BacterialRNA, MessengerSingle-Cell AnalysisSpatio-Temporal AnalysisVirulenceFimbriae ProteinsFlagellinPyocinsRNA, BacterialRNA, Messenger

Identifiers

PMID34385369
PMCPMC8454218
OpenAlexW3192380610

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.