Deep Learning-Enabled Resolution-Enhancement in Mini- and Regular Microscopy for Biomedical Imaging.

Artificial intelligence algorithms that aid mini-microscope imaging are attractive for numerous applications. In this paper, we optimize artificial intelligence techniques to provide clear, and natural biomedical imaging. We demonstrate that a deep learning-enabled super-resolution method can significantly enhance the spatial resolution of mini-microscopy and regular-microscopy. This data-driven approach trains a generative adversarial network to transform low-resolution images into super-resolved ones. Mini-microscopic images and regular-microscopic images acquired with different optical microscopes under various magnifications are collected as our experimental benchmark datasets. The only input to this generative-adversarial-network-based method are images from the datasets down-sampled by the Bicubic interpolation. We use independent test set to evaluate this deep learning approach with other deep learning-based algorithms through qualitative and quantitative comparisons. To clearly present the improvements achieved by this generative-adversarial-network-based method, we zoom into the local features to explore and highlight the qualitative differences. We also employ the peak signal-to-noise ratio and the structural similarity, to quantitatively compare alternative super-resolution methods. The quantitative results illustrate that super-resolution images obtained from our approach with interpolation parameter