Evidence mapPaperPMID 34436070Full record

ArticleBiosensors2021

Development of a Point-of-Care System Based on White Light Reflectance Spectroscopy: Application in CRP Determination.

Dimitra Tsounidi, Georgios Koukouvinos, Vasilios Christianidis, Evangelia Legaki, Vasiliki Giogli, Konstantina Panagiotopoulou, Styliani Taka, Zoi Ekaterinidi, Sotirios Kakabakos, Ioannis Raptis and 1 more

Abstract read
In one paragraph

Article in Biosensors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Dimitra TsounidiImmunoassay/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Centre for Scientific Research ''Demokritos", 15310 Aghia Paraskevi, Greece.
Georgios KoukouvinosImmunoassay/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Centre for Scientific Research ''Demokritos", 15310 Aghia Paraskevi, Greece.ORCID 0000-0002-2418-1876
Vasilios ChristianidisThetaMetrisis S.A., Polydefkous 14, 12243 Egaleo, Greece.ORCID 0000-0002-8083-6021
Evangelia LegakiSTARTBIO Molecular Diagnostics and Biotechnology Services P.C., 11527 Athens, Greece.ORCID 0000-0003-4261-2745
Vasiliki GiogliSTARTBIO Molecular Diagnostics and Biotechnology Services P.C., 11527 Athens, Greece.
Konstantina PanagiotopoulouSTARTBIO Molecular Diagnostics and Biotechnology Services P.C., 11527 Athens, Greece.
Styliani TakaSTARTBIO Molecular Diagnostics and Biotechnology Services P.C., 11527 Athens, Greece.ORCID 0000-0001-7607-8005
Zoi EkaterinidiSoftware Competitiveness International GmbH, 64293 Darmstadt, Germany.
Sotirios KakabakosImmunoassay/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Centre for Scientific Research ''Demokritos", 15310 Aghia Paraskevi, Greece.ORCID 0000-0002-0043-3119
Ioannis RaptisThetaMetrisis S.A., Polydefkous 14, 12243 Egaleo, Greece.
Panagiota PetrouImmunoassay/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Centre for Scientific Research ''Demokritos", 15310 Aghia Paraskevi, Greece.

Funding

Horizon 2020 824920Stavros Niarchos Foundation Industrial Scholarships
6 · The paper itself

Abstract

The development of methods and miniaturized systems for fast and reliable quantitative determinations at the Point-of-Care is a top challenge and priority in diagnostics. In this work, a compact bench-top system, based on White Light Reflectance Spectroscopy, is introduced and evaluated in an application with high clinical interest, namely the determination of C-Reactive protein (CRP) in human blood samples. The system encompassed all the necessary electronic and optical components for the performance of the assay, while the dedicated software provided the sequence and duration of assay steps, the reagents flow rate, the real-time monitoring of sensor response, and data processing to deliver in short time and accurately the CPR concentration in the sample. The CRP assay included two steps, the first comprising the binding of sample CRP onto the chip immobilized capture antibody and the second the reaction of the surface immunosorbed CRP molecules with the detection antibody. The assay duration was 12 min and the dynamic range was from 0.05 to 200 μg/mL, covering both normal values and acute inflammation incidents. There was an excellent agreement between CRP values determined in human plasma samples using the developed device with those received for the same samples by a standard diagnostic laboratory method.

Indexed as

Biosensing TechniquesPoint-of-Care SystemsAntibodiesC-Reactive ProteinEquipment DesignHumansLightLimit of DetectionSpectrum AnalysisAntibodiesC-Reactive ProteinC-Reactive proteinlabel-free detectionpoint-of carewhite light reflectance spectroscopy

Identifiers

PMID34436070
PMCPMC8394791

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.