ArticleBiosensors2021
A Rapid Single-Cell Antimicrobial Susceptibility Testing Workflow for Bloodstream Infections.
Article in Biosensors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Rapid and robust diagnosis of bloodstream infections by single-cell analysis.Science advances · 2026Article
- Recent Advances in Bacterial Separation and Enrichment from Blood for the Diagnosis of Bloodstream Infections.Sensors (Basel, Switzerland) · 2026Review
- Diagnostic Performance of Droplet Digital PCR for Detecting Pathogens and Resistance Genes in Patients with Suspected Bloodstream Infections.Infection and drug resistance · 2026Article
- Microfluidic point-of-care testing for antimicrobial resistance: advances, strategies, and One Health perspectives.Science in One Health · 2026Review
- Plug-and-Play Centrifuge-Only Device for Rapid Sepsis Diagnosis.Advanced healthcare materials · 2025Article
- Culture-free detection of bacteria from blood for rapid sepsis diagnosis.NPJ digital medicine · 2025Article
- Simple dual filter workflow for facilitating blood culture-free and sensitive detection of pathogenic bacteria from blood.Scientific reports · 2025Article
- Isolation and identification of bacteria from blood within 12 h using standard laboratory equipment.Scientific reports · 2025Article
- Label-free rapid antimicrobial susceptibility testing with machine-learning based dynamic holographic laser speckle imaging.Biosensors & bioelectronics · 2025Article
- Development of a Mass-Producible Microfluidic Device for Single and Bulk Mycobacteria Investigations.Biosensors · 2025Article
- Distinguishing mutants that resist drugs via different mechanisms by examining fitness tradeoffs.eLife · 2024Article
- Distinguishing mutants that resist drugs via different mechanisms by examining fitness tradeoffs.bioRxiv : the preprint server for biology · 2024Article
- Microfluidic technologies for advanced antimicrobial susceptibility testing.Biomicrofluidics · 2024Review
- Rapid Minimum Inhibitory Concentration (MIC) Analysis Using Lyophilized Reagent Beads in a Novel Multiphase, Single-Vessel Assay.Antibiotics (Basel, Switzerland) · 2023Article
- Design and Application of Portable Centrifuge Inspired by a Hand-Powered Spinning Top.Micromachines · 2023Article
- Single-cell scattering and auto-fluorescence-based fast antibiotic susceptibility testing for gram-negative and gram-positive bacteria.Frontiers in microbiology · 2023Article
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- Diagnosis of Bloodstream Infections: An Evolution of Technologies towards Accurate and Rapid Identification and Antibiotic Susceptibility Testing.Antibiotics (Basel, Switzerland) · 2022Review
- Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters.Microorganisms · 2021Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Bloodstream infections are a significant cause of morbidity and mortality worldwide. The rapid initiation of effective antibiotic treatment is critical for patients with bloodstream infections. However, the diagnosis of bloodborne pathogens is largely complicated by the matrix effect of blood and the lengthy blood tube culture procedure. Here we report a culture-free workflow for the rapid isolation and enrichment of bacterial pathogens from whole blood for single-cell antimicrobial susceptibility testing (AST). A dextran sedimentation step reduces the concentration of blood cells by 4 orders of magnitude in 20-30 min while maintaining the effective concentration of bacteria in the sample. Red blood cell depletion facilitates the downstream centrifugation-based enrichment step at a sepsis-relevant bacteria concentration. The workflow is compatible with common antibiotic-resistant bacteria and does not influence the minimum inhibitory concentrations. By applying a microfluidic single-cell trapping device, we demonstrate the workflow for the rapid determination of bacterial infection and antimicrobial susceptibility testing at the single-cell level. The entire workflow from blood to categorical AST result can be completed in less than two hours.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.