Evidence map›Paper›PMID 34458065›Full record

Article3 Biotech2021

Selection and validation of reference genes for RT-qPCR analysis in

Zhiqiang Wang, Fangqin Yu, Dingding Shi, Ying Wang, Feng Xu, Shaohua Zeng

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in 3 Biotech, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. Genome-Wide Analysis ofPlants (Basel, Switzerland) · 2025
    Article
  2. Article
  3. Heliyon · 2024
    Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Zhiqiang WangCollege of Horticulture and Gardening, Yangtze University, Jingzhou, 434025 China.
Fangqin YuCollege of Life Sciences, Gannan Normal University, Ganzhou, 341000 Jiangxi China.
Dingding ShiGuangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650 China.
Ying WangGuangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650 China.
Feng XuCollege of Horticulture and Gardening, Yangtze University, Jingzhou, 434025 China.
Shaohua ZengCollege of Life Sciences, Gannan Normal University, Ganzhou, 341000 Jiangxi China.ORCID 0000-0003-4018-7933
Gannan Normal University · CNSouth China Botanical Garden · CNYangtze University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene expression valuated by reverse transcription-quantitative PCR (RT-qPCR) are often applied to study the gene function. To obtain accurate and reliable results, the usage of stable reference genes is essential for RT-qPCR analysis. The traditional southern Chinese medicinal herb, SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-021-02954-x.

Indexed as

Desmodium styracifolium MerrMedicinal herbReference geneTissues

Identifiers

PMID34458065
PMCPMC8353030
OpenAlexW3191933809

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.