Evidence map›Paper›PMID 34526090›Full record

ArticleJournal of ovarian research2021

Effects of single and multiple transplantations of human umbilical cord mesenchymal stem cells on the recovery of ovarian function in the treatment of premature ovarian failure in mice.

Xiaodan Lv, Chunyi Guan, Ying Li, Xing Su, Lu Zhang, Xueqin Wang, Hong-Fei Xia, Xu Ma

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 3 pooled it
7.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 3 syntheses or guidelines pooled it, 50 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Xiaodan Lv *Reproductive and Genetic Center of the National Research Institute for Family Planning, Beijing, 100081, China.
Chunyi Guan *Reproductive and Genetic Center of the National Research Institute for Family Planning, Beijing, 100081, China.
Ying LiReproductive and Genetic Center of the National Research Institute for Family Planning, Beijing, 100081, China.
Xing SuReproductive and Genetic Center of the National Research Institute for Family Planning, Beijing, 100081, China.
Lu ZhangReproductive and Genetic Center of the National Research Institute for Family Planning, Beijing, 100081, China.
Xueqin WangReproductive and Genetic Center of the National Research Institute for Family Planning, Beijing, 100081, China.
Hong-Fei XiaReproductive and Genetic Center of the National Research Institute for Family Planning, Beijing, 100081, China. hongfeixia@126.com.
Xu MaReproductive and Genetic Center of the National Research Institute for Family Planning, Beijing, 100081, China. genetic@263.net.cn.
Chinese Academy of Medical Sciences & Peking Union Medical College · CNPeking Union Medical College Hospital · CNNational Health and Family Planning Commission · CN

Funding

National Natural Science Foundation of China No.81771590National Research Institute for Family Planning No. 2020GJZ02Special Funds for Fundamental Research Expenses of Central Universities 3332020111
6 · The paper itself

Abstract

backgroundCurrently, there is no effective treatment for premature ovarian failure (POF), and stem cell therapy is considered the most promising treatment. Human umbilical cord blood mesenchymal stem cells (hUC-MSCs) have shown good regenerative ability in various diseases, including POF; however, their underlying mechanism and dosage for POF treatment remain unclear. This study aimed to compare the effect of single and multiple injections of hUC-MSCs on ovarian function repair in chemotherapy-induced POF.

methodsFemale mice were intraperitoneally injected with 30 mg/kg busulfan and 120 mg/kg cyclophosphamide (CTX) to induce POF. In the single hUC-MSC injection group, hUC-MSCs were transplanted into mice D7 after CTX and busulfan administration, while in the multiple injection group, hUC-MSCs were transplanted on D7, D14, and D21 after CTX and busulfan administration. We evaluated the ovarian morphology, fertility, follicle-stimulating hormone and estradiol concentrations, follicle count, POF model, and cell transplantation results. In addition, real-time polymerase chain reaction, immunohistochemistry, and miRNA and mRNA chips were used to evaluate the effect of the cell therapy.

resultsOvary size, number of follicle at all developmental stages, and fertility were significantly reduced in the POF group compared with the control. Under hUC-MSC treatment, the ovarian morphology and follicle count were significantly restored, and fertility was significantly increased. By comparing the single and multiple hUC-MSC injection groups, we found that the anti-Müllerian hormone and Ki-67 levels were significantly increased in the multiple hUC-MSC group on D60 after chemotherapy. The expression of stimulating hormone receptors, inhibin α, and inhibin β was significantly restored, and the therapeutic effect was superior to that of the single hUC-MSC injection group.

conclusionThese results indicate that hUC-MSCs can restore the structure of injured ovarian tissue and its function in chemotherapy-induced POF mice and ameliorate fertility. Multiple hUC-MSC transplantations have a better effect on the recovery of ovarian function than single hUC-MSC transplantation in POF.

Indexed as

AnimalsDisease Models, AnimalFemaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationMiceOvarian FolliclePrimary Ovarian InsufficiencyUmbilical CordhUC-MSCsOvarian functionPremature ovarian failureTransplantation

Identifiers

PMID34526090
PMCPMC8442267
OpenAlexW3110367443

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.