Evidence mapPaperPMID 34576986Full record

ReviewMolecules (Basel, Switzerland)2021

DNA Dyes-Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods.

Anna Ligasová, Karel Koberna

Abstract readComparative StudyReview
In one paragraph

Review in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
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  9. Review
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  12. A BODIPY-Hoechst conjugate for nuclear DNA staining.Journal of porphyrins and phthalocyanines
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Anna LigasováInstitute of Molecular and Translational Medicine Dentistry, Faculty of Medicine and Dentistry and Czech Advanced Technology Research Institute, Palacký University in Olomouc, Hněvotínská 5, 779 00 Olomouc, Czech Republic.ORCID 0000-0002-1564-3768
Karel KobernaInstitute of Molecular and Translational Medicine Dentistry, Faculty of Medicine and Dentistry and Czech Advanced Technology Research Institute, Palacký University in Olomouc, Hněvotínská 5, 779 00 Olomouc, Czech Republic.

Funding

European Regional Development Fund project ENOCH, grant number CZ.02.1.01/0.0/0.0/16_019/0000868Ministerstvo Školství, Mládeže a Tělovýchovy project EATRIS-CZ, grant number LM2018133Technology Agency of the Czech Republic TN01000013
6 · The paper itself

Abstract

Cell quantification is widely used both in basic and applied research. A typical example of its use is drug discovery research. Presently, plenty of methods for cell quantification are available. In this review, the basic techniques used for cell quantification, with a special emphasis on techniques based on fluorescent DNA dyes, are described. The main aim of this review is to guide readers through the possibilities of cell quantification with various methods and to show the strengths and weaknesses of these methods, especially with respect to their sensitivity, accuracy, and length. As these methods are frequently accompanied by an analysis of cell proliferation and cell viability, some of these approaches are also described.

Indexed as

Fluorescent DyesBiological AssayCell CountCell ProliferationCell SurvivalDNADNAFluorescent Dyescell metabolismcell quantificationDNA dyesenzymatic conversion of substrate

Identifiers

PMID34576986
PMCPMC8465179

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.