Evidence map›Paper›PMID 34580200›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2021

Culturing patient-derived malignant hematopoietic stem cells in engineered and fully humanized 3D niches.

Andrés García-García, Thibaut Klein, Gordian Born, Morgane Hilpert, Arnaud Scherberich, Claudia Lengerke, Radek C Skoda, Paul E Bourgine, Ivan Martin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 37 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. 3DACS biomaterials science & engineering · 2026
    Review
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Review
  14. Article
  15. Review
  16. Review
  17. Osteoblast Lineage Support of Hematopoiesis in Health and Disease.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2022
    Review
  18. Review
  19. Article
  20. Engineering complexity in human tissue models of cancer.Advanced drug delivery reviews · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Andrés García-GarcíaTissue Engineering, Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland; andres.garcia-garcia@unibas.ch ivan.martin@usb.ch.ORCID 0000-0002-8797-649X
Thibaut KleinTissue Engineering, Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland.
Gordian BornTissue Engineering, Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland.
Morgane HilpertTissue Engineering, Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland.
Arnaud ScherberichTissue Engineering, Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland.ORCID 0000-0003-3468-6955
Claudia LengerkeStem Cells and Hematopoiesis, Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland.
Radek C SkodaExperimental Hematology, Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland.
Paul E BourgineLaboratory for Cell, Tissue, and Organ Engineering, Department of Clinical Sciences, Wallenberg Center for Molecular Medicine, Lund University, 22100 Lund, Sweden.ORCID 0000-0002-7639-6844
Ivan MartinTissue Engineering, Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland; andres.garcia-garcia@unibas.ch ivan.martin@usb.ch.ORCID 0000-0001-6493-0432
University of Basel · CHLund University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human malignant hematopoietic stem and progenitor cells (HSPCs) reside in bone marrow (BM) niches, which remain challenging to explore due to limited in vivo accessibility and constraints with humanized animal models. Several in vitro systems have been established to culture patient-derived HSPCs in specific microenvironments, but they do not fully recapitulate the complex features of native bone marrow. Our group previously reported that human osteoblastic BM niches (O-N), engineered by culturing mesenchymal stromal cells within three-dimensional (3D) porous scaffolds under perfusion flow in a bioreactor system, are capable of maintaining, expanding, and functionally regulating healthy human cord blood-derived HSPCs. Here, we first demonstrate that this 3D O-N can sustain malignant CD34

Indexed as

AnimalsAntigens, CD34Bone MarrowCell DifferentiationCell ProliferationHematopoietic Stem CellsHumansLeukemia, Myeloid, AcuteMesenchymal Stem CellsOsteoblastsStem Cell NicheStromal Vascular FractionTissue ScaffoldsTumor MicroenvironmentAntigens, CD343D perfusion bioreactorsAML/MPNengineering stem cell nichesosteoblastic nichestromal-vascular niche

Identifiers

PMID34580200
PMCPMC8501773
OpenAlexW3201788962

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.