Evidence map›Paper›PMID 34586382›Full record

ArticleG3 (Bethesda, Md.)2021

Ubc1 turnover contributes to the spindle assembly checkpoint in Saccharomyces cerevisiae.

Heather E Arsenault, Julie M Ghizzoni, Cassandra M Leech, Anne R Diers, Stephane Gesta, Vivek K Vishnudas, Niven R Narain, Rangaprasad Sarangarajan, Jennifer A Benanti

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in G3 (Bethesda, Md.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Heather E ArsenaultDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Julie M GhizzoniDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Cassandra M LeechDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-4741-9568
Anne R DiersBERG, Framingham, MA 01710, USA.
Stephane GestaBERG, Framingham, MA 01710, USA.
Vivek K VishnudasBERG, Framingham, MA 01710, USA.
Niven R NarainBERG, Framingham, MA 01710, USA.
Rangaprasad SarangarajanBERG, Framingham, MA 01710, USA.
Jennifer A BenantiDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Framingham State University · USUniversity of Massachusetts Chan Medical School · US

Funding

Molecular Mechanisms of Cell Cycle ControlR35GM136280 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Jennifer A Benanti · 2020 to 2026
$4.8M
NIGMS NIH HHS R35 GM136280
6 · The paper itself

Abstract

The spindle assembly checkpoint protects the integrity of the genome by ensuring that chromosomes are properly attached to the mitotic spindle before they are segregated during anaphase. Activation of the spindle checkpoint results in inhibition of the Anaphase-Promoting Complex (APC), an E3 ubiquitin ligase that triggers the metaphase-anaphase transition. Here, we show that levels of Ubc1, an E2 enzyme that functions in complex with the APC, modulate the response to spindle checkpoint activation in Saccharomyces cerevisiae. Overexpression of Ubc1 increased resistance to microtubule poisons, whereas Ubc1 shut-off sensitized cells. We also found that Ubc1 levels are regulated by the spindle checkpoint. Checkpoint activation or direct APC inhibition led to a decrease in Ubc1 levels, charging, and half-life. Additionally, stabilization of Ubc1 prevented its down-regulation by the spindle checkpoint and increased resistance to checkpoint-activating drugs. These results suggest that down-regulation of Ubc1 in response to spindle checkpoint signaling is necessary for a robust cell cycle arrest.

Indexed as

Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsAnaphaseAnaphase-Promoting Complex-CyclosomeCdc20 ProteinsCell Cycle ProteinsMitosisM Phase Cell Cycle CheckpointsSpindle ApparatusUbiquitin-Conjugating EnzymesAnaphase-Promoting Complex-CyclosomeCdc20 ProteinsCell Cycle ProteinsSaccharomyces cerevisiae ProteinsUBC1 protein, S cerevisiaeUbiquitin-Conjugating Enzymesanaphase-promoting complexmitosisspindle assembly checkpoint

Identifiers

PMID34586382
PMCPMC8664427
OpenAlexW3201927870

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.