Evidence map›Paper›PMID 34597676›Full record

ArticleExperimental eye research2021

Quantification and image-derived phenotyping of retinal ganglion cell nuclei in the nee mouse model of congenital glaucoma.

Carly J van der Heide, Kacie J Meyer, Adam Hedberg-Buenz, Danielle Pellack, Nicholas Pomernackas, Hannah E Mercer, Michael G Anderson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Carly J van der HeideDepartment of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Rd., Iowa City, IA, 52242, USA. Electronic address: carly-vanderheide@uiowa.edu.
Kacie J MeyerDepartment of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Rd., Iowa City, IA, 52242, USA. Electronic address: kacie-meyer@uiowa.edu.
Adam Hedberg-BuenzDepartment of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Rd., Iowa City, IA, 52242, USA; VA Center for the Prevention and Treatment of Visual Loss, Iowa City VA Health Care System, 601 Hwy 6 West (151), Iowa City, IA, 52246, USA. Electronic address: adam-hedberg-buenz@uiowa.edu.
Danielle PellackDepartment of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Rd., Iowa City, IA, 52242, USA. Electronic address: danielle.pellack@gmail.com.
Nicholas PomernackasDepartment of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Rd., Iowa City, IA, 52242, USA. Electronic address: nicholas-pomernackas@uiowa.edu.
Hannah E MercerDepartment of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Rd., Iowa City, IA, 52242, USA. Electronic address: hbaker0123@gmail.com.
Michael G AndersonDepartment of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Rd., Iowa City, IA, 52242, USA; VA Center for the Prevention and Treatment of Visual Loss, Iowa City VA Health Care System, 601 Hwy 6 West (151), Iowa City, IA, 52246, USA; Department of Ophthalmology and Visual Sciences, University of Iowa, 200 Hawkins Dr., Iowa City, IA, 52242, USA. Electronic address: michael-g-anderson@uiowa.edu.
University of Iowa · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007337 · NIGMS · UNIVERSITY OF IOWA · PI GEYER, PAMELA K., LENTZ, STEVEN R · 1985 to 2020
$15.9M
Stem Cell and Organoid CoreP30EY025580 · NEI · UNIVERSITY OF IOWA · PI Budd A Tucker · 2016 to 2026
$6.9M
Diabetes Research Training Program Diversity SupplementT32DK112751 · NIDDK · UNIVERSITY OF IOWA · PI Andrew W Norris · 2017 to 2026
$4.8M
Fatty Acid Defects & Oxidative Stress Modulate Islet Function in Cystic FibrosisR01DK097820 · NIDDK · UNIVERSITY OF IOWA · PI NORRIS, ANDREW W, UC, ALIYE · 2012 to 2016
$1.9M
Therapy of Nocturnal Intraocular Pressure Elevation Causing Glaucoma ProgressionI01RX001481 · VA · IOWA CITY VA MEDICAL CENTER · PI ANDERSON, MICHAEL G · 2015 to 2019
–
NEI NIH HHS P30 EY025580NIDDK NIH HHS R01 DK097820NIDDK NIH HHS T32 DK112751NIGMS NIH HHS T32 GM007337RRD VA I01 RX001481
6 · The paper itself

Abstract

The nee mouse model exhibits characteristic features of congenital glaucoma, a common cause of childhood blindness. The current study of nee mice had two components. First, the time course of neurodegeneration in nee retinal flat-mounts was studied over time using a retinal ganglion cell (RGC)-marker, BRN3A; a pan-nuclear marker, TO-PRO-3; and H&E staining. Based on segmentation of nuclei using ImageJ and RetFM-J, this analysis identified a rapid loss of BRN3A

Indexed as

AnimalsCell CountCell NucleusDisease Models, AnimalGlaucomaMiceMice, Mutant StrainsRetinal Ganglion CellsTomography, Optical CoherenceCell counterCell nucleusDisplaced amacrine cellsGlaucomaImage segmentationMouseRetinaRetinal ganglion cells

Identifiers

PMID34597676
PMCPMC8608716
OpenAlexW3201886417

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.