ArticleiScience2021
The gut microbiota instructs the hepatic endothelial cell transcriptome.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Monocolonization withMicrobiology spectrum · 2026Article
- Intestinal decontamination with rifaximin attenuates LSEC dysfunction and liver fibrosis in mice.PloS one · 2026Article
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- The gut microbiota in thrombosis.Nature reviews. Cardiology · 2025Review
- The potential roles of gut microbiome in porto-sinusoidal vascular disease: an under-researched crossroad.Frontiers in microbiology · 2025Review
- Myeloid-Mas Signaling Modulates Pathogenic Crosstalk among MYCAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Comprehensive bibliometric and visualized analysis of research on gut-liver axis published from 1998 to 2022.Heliyon · 2024Article
- Gut microbiota-gonadal axis: the impact of gut microbiota on reproductive functions.Frontiers in immunology · 2024Review
- Article
- Gut Microbiota and the Microvasculature.Cold Spring Harbor perspectives in medicine · 2023Review
- Blood Coagulation and Beyond: Position Paper from the Fourth Maastricht Consensus Conference on Thrombosis.Thrombosis and haemostasis · 2023Article
- Sterility testing of germ-free mouse colonies.Frontiers in immunology · 2023Review
- Role of Intestinal Microbes in Chronic Liver Diseases.International journal of molecular sciences · 2022Review
- Microbiota-derived tryptophan metabolites in vascular inflammation and cardiovascular disease.Amino acids · 2022Review
- Article
- The Gut Microbiome and Nonalcoholic Steatohepatitis.Gastroenterology & hepatology · 2022Article
- Article
- Editorial: Impact of the gut microbiota on cardiovascular medicine.Frontiers in medicine · 2022Article
- The relationship between the number of stenotic coronary arteries and the gut microbiome in coronary heart disease patients.Frontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
17 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gut microbiota affects remote organ functions but its impact on organotypic endothelial cell (EC) transcriptomes remains unexplored. The liver endothelium encounters microbiota-derived signals and metabolites via the portal circulation. To pinpoint how gut commensals affect the hepatic sinusoidal endothelium, a magnetic cell sorting protocol, combined with fluorescence-activated cell sorting, was used to isolate hepatic sinusoidal ECs from germ-free (GF) and conventionally raised (CONV-R) mice for transcriptome analysis by RNA sequencing. This resulted in a comprehensive map of microbiota-regulated hepatic EC-specific transcriptome profiles. Gene Ontology analysis revealed that several functional processes in the hepatic endothelium were affected. The absence of microbiota influenced the expression of genes involved in cholesterol flux and angiogenesis. Specifically, genes functioning in hepatic endothelial sphingosine metabolism and the sphingosine-1-phosphate pathway showed drastically increased expression in the GF state. Our analyses reveal a prominent role for the microbiota in shaping the transcriptional landscape of the hepatic endothelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.