ArticleNature communications2021
Circa-SCOPE: high-throughput live single-cell imaging method for analysis of circadian clock resetting.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- A High-Throughput Live Imaging Platform to Investigate Circuit-Dependent Regulation of Circadian Rhythms in Brain Tissue.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Transcriptional noise sets fundamental limits to decoding circadian clock phase from single-cell RNA snapshots.iScience · 2026Article
- The role and implications of mammalian cellular circadian entrainment.FEBS letters · 2026Review
- Steroid hormone receptors through Cry2 are key players in the circadian clock response to serum.Nature communications · 2025Article
- The time is now: accounting for time-of-day effects to improve reproducibility and translation of metabolism research.Nature metabolism · 2025Review
- Parameterized resetting model captures dose-dependent entrainment of the mouse circadian clock.Nature communications · 2025Article
- High-Throughput Single-Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC).Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Dysregulation of the molecular clock by blood-borne factors in Alzheimer's disease patients.Journal of neurology · 2025Observational
- Circadian period is compensated for repressor protein turnover rates in single cells.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Circadian regulation of liver function: from molecular mechanisms to disease pathophysiology.Nature reviews. Gastroenterology & hepatology · 2023Review
- Circadian regulation of liver metabolism: experimental approaches in human, rodent, and cellular models.American journal of physiology. Cell physiology · 2023Review
- Mechanical control of the mammalian circadian clock via YAP/TAZ and TEAD.The Journal of cell biology · 2023Article
- Cyclical dermal micro-niche switching governs the morphological infradian rhythm of mouse zigzag hair.Nature communications · 2023Article
- Singularity response reveals entrainment properties in mammalian circadian clock.Nature communications · 2023Article
- Input integration by the circadian clock exhibits nonadditivity and fold-change detection.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Different levels of circadian (de)synchrony -- where does it hurt?F1000Research · 2022Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circadian clocks are self-sustained and cell-autonomous oscillators. They respond to various extracellular cues depending on the time-of-day and the signal intensity. Phase Transition Curves (PTCs) are instrumental in uncovering the full repertoire of responses to a given signal. However, the current methodologies for reconstructing PTCs are low-throughput, laborious, and resource- and time-consuming. We report here the development of an efficient and high throughput assay, dubbed Circadian Single-Cell Oscillators PTC Extraction (Circa-SCOPE) for generating high-resolution PTCs. This methodology relies on continuous monitoring of single-cell oscillations to reconstruct a full PTC from a single culture, upon a one-time intervention. Using Circa-SCOPE, we characterize the effects of various pharmacological and blood-borne resetting cues, at high temporal resolution and a wide concentration range. Thus, Circa-SCOPE is a powerful tool for comprehensive analysis and screening for circadian clocks' resetting cues, and can be valuable for basic as well as translational research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.