Evidence map›Paper›PMID 34629821›Full record

ArticleWorld journal of gastroenterology2021

Exosomal microRNA-588 from M2 polarized macrophages contributes to cisplatin resistance of gastric cancer cells.

Hai-Yan Cui, Jian-Sheng Rong, Ju Chen, Jie Guo, Jia-Qin Zhu, Mei Ruan, Rong-Rong Zuo, Shuang-Shuang Zhang, Jun-Mei Qi, Bao-Hua Zhang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
2.9field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Hai-Yan CuiDepartment of Pathology, The Fourth People's Hospital of Zibo City, Zibo 255000, Shandong Province, China.
Jian-Sheng RongDepartment of Pathology, Zibo Central Hospital, Zibo 255036, Shandong Province, China.
Ju ChenDepartment of Ultrasound Medicine, Zibo Central Hospital, Zibo 255036, Shandong Province, China.
Jie GuoDepartment of Health, The Fourth People's Hospital of Zibo City, Zibo 255000, Shandong Province, China.
Jia-Qin ZhuDepartment of Gastroenterology, The Fourth People's Hospital of Zibo City, Zibo 255000, Shandong Province, China.
Mei RuanDepartment of Oncology, The Fourth People's Hospital of Zibo City, Zibo 255000, Shandong Province, China.
Rong-Rong ZuoDepartment of Pathology, The Fourth People's Hospital of Zibo City, Zibo 255000, Shandong Province, China.
Shuang-Shuang ZhangDepartment of Pathology, The Fourth People's Hospital of Zibo City, Zibo 255000, Shandong Province, China.
Jun-Mei QiDepartment of Pathology, The Fourth People's Hospital of Zibo City, Zibo 255000, Shandong Province, China.
Bao-Hua ZhangDepartment of Pathology, Zibo Central Hospital, Zibo 255036, Shandong Province, China. zhangbaohua314@163.com.
The Fourth People's Hospital of Zibo City · CNCentral Hospital of Zibo · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGastric cancer is a prevalent malignant cancer with a high incidence and significantly affects the health of modern people globally. Cisplatin (DDP) is one of the most common and effective chemotherapies for patients with gastric cancer, but DDP resistance remains a severe clinical challenge.

aimTo explore the function of M2 polarized macrophages-derived exosomal microRNA (miR)-588 in the modulation of DDP resistance of gastric cancer cells.

methodsM2 polarized macrophages were isolated and identified by specific markers using flow cytometry analysis. The exosomes from M2 macrophages were identified by transmission electron microscopy and related markers. The uptake of the PKH67-labelled M2 macrophages-derived exosomes was detected in SGC7901 cells. The function and mechanism of exosomal miR-588 from M2 macrophages in the modulation of DDP resistance of gastric cancer cells was analyzed by CCK-8 assay, apoptosis analysis, colony formation assay, Western blot analysis, qPCR analysis, and luciferase reporter assay in SGC7901 and SGC7901/DDP cells, and by tumorigenicity analysis in nude mice.

resultsM2 polarized macrophages were isolated from mouse bone marrow stimulated with interleukin (IL)-13 and IL-4. Co-cultivation of gastric cancer cells with M2 polarized macrophages promoted DDP resistance. M2 polarized macrophages-derived exosomes could transfer in gastric cancer cells to enhance DDP resistance. Exosomal miR-588 from M2 macrophages contributed to DDP resistance of gastric cancer cells. miR-588 promoted DDP-resistant gastric cancer cell growth

conclusionIn conclusion, we uncovered that exosomal miR-588 from M2 macrophages contributes to DDP resistance of gastric cancer cells by partly targeting CYLD. miR-588 may be applied as a potential therapeutic target for the treatment of gastric cancer.

Indexed as

MicroRNAsStomach NeoplasmsAnimalsCell Line, TumorCisplatinDrug Resistance, NeoplasmGene Expression Regulation, NeoplasticHumansMacrophagesMiceMice, NudeCisplatinMicroRNAsMIRN588 microRNA, humanCisplatin resistanceCylindromatosisExosomeGastric cancerM2 polarized macrophagesmiR-588

Identifiers

PMID34629821
PMCPMC8476330
OpenAlexW3202860484

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.