ArticleCell2021
Biosensors based on peptide exposure show single molecule conformations in live cells.
Article in Cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
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- Directly adopting inverse biosensors to image live cell enzyme activities in nanodomains.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- RhoG, Rac1 and Cdc42 cooperation in cell protrusion revealed by multiplexed optogenetics and biosensor imaging.bioRxiv : the preprint server for biology · 2026Article
- Steric confinement-induced emission probe for monitoring protein conformations in live cells.Communications chemistry · 2026Article
- Activation of AKT via a dual mechanism enhances the susceptibility of melanoma cells to glucose deprivation.Cell death & disease · 2025Article
- Genetically encoded mechano-sensors with versatile readouts and compact size.bioRxiv : the preprint server for biology · 2025Article
- Direct Quantification of Protein-Protein Interactions in Living Bacterial Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Conditional Control of Benzylguanine Reaction with the Self-Labeling SNAP-tag Protein.Bioconjugate chemistry · 2025Article
- Harnessing the Power of Plasmonics forACS photonics · 2025Review
- Full-length GSDME mediates pyroptosis independent from cleavage.Nature cell biology · 2024Article
- Essential Roles of PIEZO1 in Mammalian Cardiovascular System: From Development to Diseases.Cells · 2024Review
- Particle-based simulations reveal two positive feedback loops allow relocation and stabilization of the polarity site during yeast mating.PLoS computational biology · 2023Article
- Spatial models of pattern formation during phagocytosis.PLoS computational biology · 2022Article
- A FRET-Based Biosensor for the Src N-Terminal Regulatory Element.Biosensors · 2022Article
- Tracking protein conformation in live cells.Nature methods · 2021Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
We describe an approach to study the conformation of individual proteins during single particle tracking (SPT) in living cells. "Binder/tag" is based on incorporation of a 7-mer peptide (the tag) into a protein where its solvent exposure is controlled by protein conformation. Only upon exposure can the peptide specifically interact with a reporter protein (the binder). Thus, simple fluorescence localization reflects protein conformation. Through direct excitation of bright dyes, the trajectory and conformation of individual proteins can be followed. Simple protein engineering provides highly specific biosensors suitable for SPT and FRET. We describe tagSrc, tagFyn, tagSyk, tagFAK, and an orthogonal binder/tag pair. SPT showed slowly diffusing islands of activated Src within Src clusters and dynamics of activation in adhesions. Quantitative analysis and stochastic modeling revealed in vivo Src kinetics. The simplicity of binder/tag can provide access to diverse proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.