ArticleBiotechnology for biofuels2021
Glutamine involvement in nitrogen regulation of cellulase production in fungi.
Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 18 citations in OpenAlex.
- The chemical blueprint of Trichoderma for next-generation microbial biostimulants.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Engineering thiamine pyrophosphate metabolism improves consolidated bioprocessing for cellulosic ethanol with inorganic nitrogen in Myceliophthora thermophila.Biotechnology for biofuels and bioproducts · 2026Article
- The effect of nitrogen availability and forms on the degradation pathway of lignocellulose by Bacillus subtilis.Bioprocess and biosystems engineering · 2026Article
- Dual RNA-Seq analysis unveils the multifaceted mechanisms ofFrontiers in microbiology · 2026Article
- Comparative transcriptome and metabolome analyses reveal the mechanism of silicon to improve stem rust resistance in oat (Avena sativa L.).Scientific reports · 2025Article
- Valorization of Coconut Shell and Blue Berries Seed Waste into Enhance Bacterial Enzyme Production: Co-fermentation and Co-cultivation Strategies.Indian journal of microbiology · 2025Article
- Screening of the Candidate Metabolite to Evaluate the Mycelium Physiological Maturation ofJournal of fungi (Basel, Switzerland) · 2024Article
- Carbon and Nitrogen Sources Influence Parasitic Responsiveness inJournal of fungi (Basel, Switzerland) · 2024Article
- Investigating the cellular functions of β-Glucosidases for synthesis of lignocellulose-degrading enzymes inEngineering microbiology · 2023Article
- LC/MS Q-TOF Metabolomic Investigation of Amino Acids and Dipeptides inJournal of agricultural and food chemistry · 2022Article
- Assessing the intracellular primary metabolic profile ofFrontiers in fungal biology · 2022Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
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Abstract
backgroundCellulase synthesized by fungi can environment-friendly and sustainably degrades cellulose to fermentable sugars for producing cellulosic biofuels, biobased medicine and fine chemicals. Great efforts have been made to study the regulation mechanism of cellulase biosynthesis in fungi with the focus on the carbon sources, while little attention has been paid to the impact and regulation mechanism of nitrogen sources on cellulase production.
resultsGlutamine displayed the strongest inhibition effect on cellulase biosynthesis in Trichoderma reesei, followed by yeast extract, urea, tryptone, ammonium sulfate and L-glutamate. Cellulase production, cell growth and sporulation in T. reesei RUT-C30 grown on cellulose were all inhibited with the addition of glutamine (a preferred nitrogen source) with no change for mycelium morphology. This inhibition effect was attributed to both L-glutamine itself and the nitrogen excess induced by its presence. In agreement with the reduced cellulase production, the mRNA levels of 44 genes related to the cellulase production were decreased severely in the presence of glutamine. The transcriptional levels of genes involved in other nitrogen transport, ribosomal biogenesis and glutamine biosynthesis were decreased notably by glutamine, while the expression of genes relevant to glutamate biosynthesis, amino acid catabolism, and glutamine catabolism were increased noticeably. Moreover, the transcriptional level of cellulose signaling related proteins ooc1 and ooc2, and the cellular receptor of rapamycin trFKBP12 was increased remarkably, whose deletion exacerbated the cellulase depression influence of glutamine.
conclusionGlutamine may well be the metabolite effector in nitrogen repression of cellulase synthesis, like the role of glucose plays in carbon catabolite repression. Glutamine under excess nitrogen condition repressed cellulase biosynthesis significantly as well as cell growth and sporulation in T. reesei RUT-C30. More importantly, the presence of glutamine notably impacted the transport and metabolism of nitrogen. Genes ooc1, ooc2, and trFKBP12 are associated with the cellulase repression impact of glutamine. These findings advance our understanding of nitrogen regulation of cellulase production in filamentous fungi, which would aid in the rational design of strains and fermentation strategies for cellulase production in industry.
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