Evidence map›Paper›PMID 34674604›Full record

ArticleAutophagy2022

Monitoring basal autophagy in the retina utilizing CAG-mRFP-EGFP-MAP1LC3B reporter mouse: technical and biological considerations.

Sriganesh Ramachandra Rao, Steven J Fliesler

Open access · bronzeAbstract read
In one paragraph

Article in Autophagy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Studying Autophagy in Microglia: Overcoming the Obstacles.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Sriganesh Ramachandra RaoDepartments of Ophthalmology and Biochemistry and Neuroscience Graduate Program, Jacobs School of Medicine and Biomedical Sciences, State University of New York- University at Buffalo, Buffalo, NY, USA.ORCID 0000-0003-1029-1051
Steven J FlieslerDepartments of Ophthalmology and Biochemistry and Neuroscience Graduate Program, Jacobs School of Medicine and Biomedical Sciences, State University of New York- University at Buffalo, Buffalo, NY, USA.ORCID 0000-0002-2557-142X
VA Western New York Healthcare System · US

Funding

University of Buffalo Clinical and Translational Science Institute - Supplement SchulyerUL1TR001412 · NCATS · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI MURPHY, TIMOTHY F · 2015 to 2024
$33.8M
ISOPRENOID METABOLISM IN THE RETINAR01EY007361 · NEI · UNIVERSITY OF SOUTH FLORIDA · PI FLIESLER, STEVEN J. · 1988 to 2015
$4.4M
BLRD Research Career Scientist Award ApplicationIK6BX005787 · VA · VA WESTERN NEW YORK HEALTHCARE SYSTEM · PI Steven J. Fliesler · 2022 to 2026
–
BLRD VA IK6 BX005787HSRD VA RCS 00-001NCATS NIH HHS UL1 TR001412NEI NIH HHS R01 EY007361
6 · The paper itself

Abstract

We describe the utility of a tandem-tagged autophagy reporter mouse model (CAG-RFP-EGFP-MAP1LC3B) in investigating basal macroautophagic/autophagic flux in the neural retina. Western blot,

Indexed as

AutophagyLipofuscinAnimalsATP-Binding Cassette TransportersDisease Models, AnimalEndothelial CellsLysosomesMiceRetinaAbca4 protein, mouseATP-Binding Cassette TransportersLipofuscinAutophagyconfocal microscopyEGFP-LC3BlipofuscinlysosomesmyoidphotoreceptorsretinaRFP-EGFP-LC3BRPE

Identifiers

PMID34674604
PMCPMC9196719
OpenAlexW3205601867

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.