Evidence map›Paper›PMID 34697383›Full record

ArticleScientific reports2021

Riluzole-induced apoptosis in osteosarcoma is mediated through Yes-associated protein upon phosphorylation by c-Abl Kinase.

Marian Raghubir, Syeda M Azeem, Rifat Hasnat, Chowdhury N Rahman, Linda Wong, Salina Yan, Yu Qi Huang, Raquel Zhagui, Angelina Blyufer, Iffat Tariq and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.9field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 1 country.

Marian RaghubirDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Syeda M AzeemPh.D. Program in Biology, The Graduate Center of City University of New York, New York, NY, 10016, USA.
Rifat HasnatDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Chowdhury N RahmanDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Linda WongDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Salina YanDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Yu Qi HuangDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Raquel ZhaguiDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Angelina BlyuferDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Iffat TariqDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Cassey TamDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Sonam LhamoDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Lucas CecilioDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Yesmin ChowdhuryDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Shraddha ChandThakuriDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA.
Shahana S MahajanDepartment of Medical Laboratory Sciences, Hunter College, City University of New York, 425 East, 25th Street, New York, NY, 10010, USA. smahajan@hunter.cuny.edu.
City University of New York · USHunter College · USThe Graduate Center, CUNY · US

Funding

Investigating drug targets and improving drug delivery for anti-cancer treatment of osteosarcomaSC1GM131929 · NIGMS · HUNTER COLLEGE · PI MAHAJAN, SHAHANA SULTANA · 2020 to 2023
$1.6M
NIGMS NIH HHS SC1 GM131929NIH HHS 1SC1GM131929- 01A1
6 · The paper itself

Abstract

Our lab has previously demonstrated Riluzole to be an effective drug in inhibiting proliferation and inducing apoptosis in both human and mouse osteosarcoma. Yes-associated protein is a transcription co-activator, known to be involved in cell proliferation or apoptosis depending on its protein partner. In the present study we investigated the role of YAP in apoptosis in osteosarcoma, we hypothesized that YAP may be activated by Riluzole to induce apoptosis in osteosarcoma. By knocking down the expression of YAP, we have demonstrated that Riluzole failed to induce apoptosis in YAP deficient osteosarcoma cells. Riluzole caused translocation of YAP from the cytoplasm to the nucleus, indicating YAP's role in apoptosis. Both Riluzole-induced phosphorylation of YAP at tyrosine 357 and Riluzole-induced apoptosis were blocked by inhibitors of c-Abl kinase. In addition, knockdown of c-Abl kinase prevented Riluzole-induced apoptosis in LM7 cells. We further demonstrated that Riluzole promoted interaction between YAP and p73, while c-Abl kinase inhibitors abolished the interaction. Subsequently, we demonstrated that Riluzole enhanced activity of the Bax promoter in a luciferase reporter assay and enhanced YAP/p73 binding on endogenous Bax promoter in a ChIP assay. Our data supports a novel mechanism in which Riluzole activates c-Abl kinase to regulate pro-apoptotic activity of YAP in osteosarcoma.

Indexed as

Apoptosisbcl-2-Associated X ProteinBone NeoplasmsCell Line, TumorCell NucleusCell SurvivalCytoplasmGene Expression Regulation, NeoplasticGene Knockdown TechniquesHumansOsteosarcomaPhosphorylationProtein TransportProto-Oncogene Proteins c-ablRiluzoleTumor Protein p73BAX protein, humanbcl-2-Associated X ProteinProto-Oncogene Proteins c-ablRiluzoleTP73 protein, humanTumor Protein p73YAP1 protein, humanYAP-Signaling Proteins

Identifiers

PMID34697383
PMCPMC8546089
OpenAlexW3209880783

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.