ArticleJournal of the American Society for Mass Spectrometry2021
Factorial Design to Optimize Matrix Spraying Parameters for MALDI Mass Spectrometry Imaging.
Article in Journal of the American Society for Mass Spectrometry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- SpotOn: A Tool to Enhance Echo Acoustic Standard Deposition for Quantitative Mass Spectrometry Imaging.Chemical & biomedical imaging · 2026Article
- Dry Deposition Strategies for MALDI-MS Imaging: Principles, Advances and Emerging Applications.Analytical science advances · 2026Review
- Mathematical Framework for Quantifying Delocalization in MALDI-MSI via a Composite Scoring Approach.ACS measurement science au · 2026Article
- The MALDI Method to Analyze the Lipid Profile, Including Cholesterol, Triglycerides and Other Lipids.Current issues in molecular biology · 2026Review
- MALDI matrix sublimation versus spray coating in the FluoMALDI imaging pipeline.Analytica chimica acta · 2025Article
- Spatial lipidomics reveals demyelination and remyelination dynamics in the mouse brain.Journal of lipid research · 2025Article
- To Spray or To Sublimate: Considerations for the Matrix Application of Three Common Positive Ion Mode Matrices.Analytical chemistry · 2025Article
- Comprehensive High-Spatial-Resolution Imaging Metabolomics Workflow for Heterogeneous Tissues.Analytical chemistry · 2025Article
- Multimodal Study of Murine Cardiovascular Remodeling: Four-Dimensional Ultrasound and Mass Spectrometry Imaging.Journal of visualized experiments : JoVE · 2025Article
- Quantitative MALDI imaging of aspirin metabolites in mouse models of triple-negative breast cancer.Theranostics · 2025Article
- MALDI MSI Protocol for Spatial Bottom-Up Proteomics at Single-Cell Resolution.Journal of proteome research · 2024Article
- Quantitative mass spectrometry imaging: therapeutics & biomolecules.Chemical communications (Cambridge, England) · 2024Review
- Applications of mass spectrometry imaging in botanical research.Advanced biotechnology · 2024Review
- Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling.Nature protocols · 2023Review
- How to sample a seizure plant: the role of the visualization spatial distribution analysis ofForensic sciences research · 2023Article
- A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids.Biomolecules · 2023Review
- Time of acquisition and high spatial resolution mass spectrometry imaging.Journal of mass spectrometry : JMS · 2023Article
- FT-ICR Mass Spectrometry Imaging at Extreme Mass Resolving Power Using a Dynamically Harmonized ICR Cell with 1ω or 2ω Detection.Analytical chemistry · 2022Article
- Advances in mass spectrometry imaging for spatial cancer metabolomics.Mass spectrometry reviewsReview
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Matrix deposition is a critical step in obtaining reproducible and spatially representative matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging data. To date, few limited studies have examined the optimization of matrix spraying parameters for maximizing analyte extraction while minimizing analyte delocalization. Herein, we present a study using automated pneumatic spraying with a heated sample-holder tray to determine an optimized model for mouse whole kidney lipid imaging using a 2,5-dihydroxybenzoic acid matrix in which the solvent flow rate, nozzle velocity, and sample heating were optimized using a two-level factorial experimental design. Parameters examined to determine the optimum model include the number of analytes, the matrix crystal size, off tissue delocalization, the signal intensity, and spray time. Our results show that sample heating using a heated tray while spraying improves the MALDI imaging performance. This improvement is possible because higher solvent flow rates can be used in the pneumatic sprayer, allowing for better sample extraction, while sample delocalization is minimized due to sample heating.
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